💡 Both albumins and globulins (e.g. antibodies) can be evaluated using the albumin globulin ratio calculator. Albumins are also responsible for keeping adequate oncotic blood pressure. It is the force that keeps the blood inside the arteries and veins. When there is a low serum albumin le...
Orphan genes inD. melanogasterhave previously been found to be under purifying selection (Palmieri et al.2014). We therefore searched for signals of selection in the set of de novo genes found inD. melanogasterby calculating the ratio of non-synonymous to synonymous codon substitution (dN/dS), ...
The Amylo-glo and GFP intensities were calculated in specific ROI using ImageJ (https://imagej.nih.gov/ij) and the mean ratio intensity of Amylo-glo was divided by the mean ratio intensity of GFP. One hundred cells in three independent experiments were analyzed. Download: Download high-res...
The negatively charged SOMAmers are designed to be complementary to the shape of the natively folded target proteins and bind the target protein tightly and specifically at a ratio of 1:1 (refs. 35,36). The method, which takes advantage of new chemically modified nucleotides, converts the ...
mitochondrial protein-coding genes. Lastly, we used a set of bioinformatic tools to estimate the ratio ω of non-synonymous (dN) to synonymous (dS) substitutions rate (ω = dN/dS) in order to identify signatures of adaptive evolution (i. e., positive selection) on PCG´s in ...
(HUM-P). IA LC-MS/MS analysis of these samples revealed large differences between the CYFRA 21-1 signals, expressed as the peak area ratio of the SP and IS, in the different matrices (Figure 2A). The maximum signal was obtained when using the least complex matrix (BSA), while the ...
(R1and R2) were determined by fitting the cross-peak intensity measured as a function of variable delay, to single-exponential decay using the Bruker Dynamic Center 2.4, available as a stand-alone ancillary software of TopSpin by Bruker.1H–15N NOE values were obtained as the ratio between ...
The threshold function converts the log 2 ratio signals from the CBS output via two logistic functions to a loss [−1,0] and gain [0-1] indicator output that is then averaged to give the genome-wide gain/loss recurrence frequency (plotted in FIG. 1). For the chromosome 6q loss ...
For imaging analysis, monoclonal FHR-1 antibodies10were coupled to Alexa Fluor 647 dye (Thermo Fisher Scientific, cat no. A20502) by mixing the dye with the antibody at a 2:1 ratio and incubating the mixture for 30 min at room temperature. Residual dye was removed using desalting columns ...
The level of ATPase activity (%) was estimated based on the ratio of the relative fluorescence of non-hydrolyzed ATP and products of its hydrolysis estimated by ImageQuant 5.2. We found that the complex does not exhibit ATPase activity. 4.7. MALDI-TOF Mass Spectrometry Analysis All mass spectra...