Poxviruses are among the largest double-stranded DNA viruses, with members such as variola virus, monkeypox virus and the vaccination strain vaccinia virus (VACV). Knowledge about the structural proteins that form the viral core has remained sparse. Whil
We found that Ankyrin repeats were the most frequently found among these viruses, followed by LRRs. These two types of repeats were found in all the families, while TPRs were mainly found in the Mimiviridae family, KELCH in Phycondaviridae and Poxviridae families, and RCC1 only in two ...
We then summed, for each immunized mouse, the titers of 50% neutralizing antibody against all pseudoviruses (pVNT50) for each group and found that the total pVNT50 in the Tri-Vac group surpassed those of the individual antigen groups (Fig. 2f). Two additional trivalent vaccines, with ...
Intrinsically disordered proteins, defying the traditional protein structure–function paradigm, are a challenge to study experimentally. Because a large part of our knowledge rests on computational predictions, it is crucial that their accuracy is high.
Animal proteins are considered to be complete, or high-quality, protein, because they have all of the essential amino acids that are needed by humans. Plant proteins are found in legumes, nuts, vegetables, seeds, some fruits and the products that are made from these foods. Plant proteins ...
nucleases, with HSV-1 UL8 being homologous to the exonuclease domain of B-family DNA polymerases24and HSV-1 UL30 having RNase H activity25. Since all viruses have both of these genes, this suggests that while these genes likely share a common ancestor, they have diverged in sequence and ...
Soluble forms of the IFN-alpha receptor, corresponding to truncated forms of the extracellular domain of the cell surface IFN-alpha receptor, have been found in human serum and in normal human urine. All known subtypes of IFN-alpha show the same antiviral antiparasitic, antiproliferative activities...
It has been for more than 30 years, since the first discovery of NEDD8 in 1992. Figure2summarized the milestone discoveries in the neddylation field, covering from target identification, validation, drug discovery to clinical trials, and clinical approval of a neddylation inhibitor for treatment of...
(around 200 nucleotides at the beginning of theMprogene and 400 nucleotides covering theORF7agene). Alignment of the sequences with those of the B.1.1.7 inoculum revealed a single non-synonymous mutation (C26916T) present in all three independent virus stocks in more than 97% of the reads...
2.3 Glycosylation in CHO cells Using glycosylation to improve performance of biopharmaceuticals has been shown to be successful. For example, Amgen found that adding extra N-glycosylation sites to its current version of EPO could improve the natural half-life of the molecule (Egrie et al., 2003...