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calculated the PCA for PKA-C in complex with the substrate PLN1–20, and identified the top component of the observed enzyme motion (PC1) which represents opening and closing of the two lobes of PKA-C. This mode of motion in PC1 (Fig. 3A) was further probed by monitoring the atomic ...
we prepared sets of EATs and a corresponding enzyme (Fig.1c)27. Using such pairs can convert an input (a non-enzymatic target protein) into enzymatic activity (Fig.1a); thereby, a gelator can be decomposed to induce a macroscopic gel–sol transition. The EAT consists of the ligand of a...
leading to sequestration of the metal. Chelation, as a typical process in the body, is a major component of enzyme functionality where a metal cofactor such as hemoglobin is involved23. The
Protein synthesisis a core component of cellular regulation and, as such, has profound effects at tissue, organ, and whole body levels.Protein synthesiscan be stimulated or inhibited in response to both endogenous and exogenous stimuli, allowing for adaptation and appropriate reactions to various stre...
Two examples of proteins present in the cell as different protein species are the well-known proteins angiotensin-converting-enzyme (ACE) (Figure 3) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Figure 4). ACE is a component of the renin-angiotensin system, which regulates blood pressure,...
Multi-enzyme complexes designed based on scaffold proteins are a current topic in molecular enzyme engineering. They have been gradually applied to increase the production of enzyme cascades, thereby achieving effective biosynthetic pathways. This paper
The major reactivity was localized to the dihydrolipoyl transacylase (E2) component of BCKD complex. By using recombinant techniques, the immunodominant BCKD-E2 epitope recognized by sera from IDCM patients was localized to amino acid (aa) sequences 116 to 134. Each of the IDCM sera that ...
However, the reductionist claim that a precise knowledge of each system component is sufficient to 13 Cooperation is the key: the CCN biological system as a gate to high complex protein superfamilies'… 245 understand its functioning, "is not applicable to biological systems either...
Formation of biofilms is a critical component of C. albicans pathogenesis [33] and the biofilm matrix provides a protective layer for the fungus against anti- fungal drugs [34]. The cargo of EVs produced by C. albicans biofilms includes key proteins and carbo- hydrates for biofilm biogenesis,...