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Boxplots: center line represents the median, lower and upper bounds represent the first and third quartiles and upper and lower whiskers extend from the top and bottom of the box by 1.4 the interquartile range. Black dots: outliers. p-values were calculated using a two-sided permutation ...
Mass spectrometry (MS) of the proximity labeling experiments was performed by the Functional Genomics Center Zurich. Specifically, the streptavidin magnetic beads were washed and resuspended in digestion buffer (10 mM Tris, 2 mM CaCl2, pH 8.2) prior to on-bead digestion using 10 ng/µL...
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Supply Center: Delivered from the Supply Center closest to you.Thermo Scientific PageRuler Plus Prestained Protein Ladder is a mixture of nine blue-, orange-, and green-stained proteins (10 to 250 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The ...
Gel bands migrating to the same molecular weight as the comparative control were excised and mass spectrometry was performed at the facilities of The Scripps Research Institute Center for Mass Spectrometry (TSRI, CA). The gel bands were destained, reduced (10 mM DTT), alkylated (55 mM ido...
This work was supported by grants from the German Research Foundation (DFG GE514/6-3 and SFB1009/A06). APK Ashraf is supported by the graduate school of the Cells-in-Motion Interfaculty Center and the Max-Planck Institute for Molecular Biomedicine (CIM/IMPRS)....
2e), NN4E mice of both sexes spent significantly less time in the center of the open field than control mice in the first 5 min of a 30-min open field test (Fig. 1d). In addition, male NN4E mice stayed longer in the closed arm of an elevated plus maze than control mice (...