This technique was originally described as being highly sensitive and specific for identifying individuals with ovarian cancer,53 although the interpretation of the spectral data has been difficult to reproduce.54 This underscores the fact that, regardless of the initial promise of a diagnostic method,...
Microglial activation plays central roles in neuroinflammatory and neurodegenerative diseases. Positron emission tomography (PET) targeting 18 kDa Translocator Protein (TSPO) is widely used for localising inflammation in vivo, but its quantitative interpretation remains uncertain. We show that TSPO expressio...
key point is to identify the technical limitations of the chosen method and design appropriate controls to enable its interpretation. Several strategies were used for gene tagging intrypanosomatidsin the pre-CRISPR-Cas9 era. Historically inLeishmaniaandT. cruzi, tagged copies of a protein of interest...
Joehanes, R. et al. Integrated genome-wide analysis of expression quantitative trait loci aids interpretation of genomic association studies.Genome Biol.18, 16 (2017). ArticlePubMedPubMed CentralGoogle Scholar Giambartolomei, C. et al. Bayesian test for colocalisation between pairs of genetic assoc...
ELISA: Enzyme-Linked Immunosorbent Assay FC: Fold change FDA: Food and Drug Administration FDR: False discovery rate HPβCD: 2-Hydroxypropyl-β-cyclodextrin NPC: Niemann-Pick disease, type C NPC1: Niemann-Pick disease, type C1 NPC2:
However, the lower protein carbonyls in pre-term infants would lead to an opposite interpretation. More information is needed on the source and fate of these and other biomarkers before drawing strong conclusions on how they reflect oxidative stress in this and other clinical situations.doi:10.1080...
MMP-12 Total Protein ELISA protocol 1. Coat the capture antibody, Dendritics cat# DDX0284, 1 hour at 3 μg/mL, 100 μL/well, 37°C in ELISA coating buffer (0.1 M carbonate/bicarbonate pH 9.55-9.65). 2. Wash with PBS wash buffer (137 mM NaCl, 2.7 mM KCl, 10 mM Sodium Phosph...
Interpretation: C-reactive protein concentration can be used to identify heart-transplant patients at increased risk of coronary artery disease and graft failure. Treatments directed at reduction of C-reactive protein concentration could improve patients' outcome.Labarrere...
J. & Rosen, C. J. New insights into osteoporosis: the bone-fat connection. J. Intern. Med. 272, 317–329 (2012). Article CAS PubMed PubMed Central Google Scholar Turcot, V. et al. Protein-altering variants associated with body mass index implicate pathways that control energy intake ...
Red: G1 strand; green: the F strand; yellow: c-terminal domain containing 4 α-helices (h2, h3, h4, and h5) and J strand. Strands (capital letters) and helices (h1-h5) are labeled. (). (For interpretation of the references to colour in this figure legend, the reader is referred...