Due to its low (∼μM) concentration (Persechini and Stemmer, 2002) in the cell, another protein, calmodulin (CaM), acts as a Ca2+sensor more than a buffer. The function of this protein in the cell is essential. By binding of Ca2+ions via EF-hand motifs, calmodulin changes its con...
Casein solutions were prepared using the following buffer system: 50 mM of sodium acetate buffer, pH 4.0–5.0; Tris–HCl buffer, pH 6.0–7.0; potassium phosphate, pH 8.0–9.0 and Glycine–NaOH, pH 10.0–11.0, respectively. The effects of metal ions on SPSFQ enzyme activity were studied by...
88222). Cell supernatants were diluted with 1/3 volume of wash buffer (20 mM imidazole, 20 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) pH 7.4, 150 mM sodium chloride (NaCl) or 20 mM imidazole, 1× PBS), and the Ni-NTA resin was added to diluted ...
The electrolyte may be continuous (a single buffer), or discontinuous, where a sample is stacked by means of a buffer discontinuity, before it enters the running gel/running buffer. The gel may be a single concentration or gradient in which pore size decreases with migration distance. In SDS...
Phages use anti-CRISPR proteins to deactivate the CRISPR–Cas system. The mechanisms for the inhibition of type I and type II systems by anti-CRISPRs have been elucidated. However, it has remained unknown how the type V CRISPR–Cas12a (Cpf1) system is in
DNA sequencing data were analysed using the cloud-based Ion Reporter system. Single-nucleotide variants and their impacts and tumour mutational burden were compared between FFPE tissues and the CA tissue-matched primary cell lines. Protein extraction Tissues and cells were lysed in lysis buffer ...
Several bacterial plant pathogens colonize their hosts through the secretion of effector proteins by a Type III protein secretion system (T3SS). The role of T3SS in bacterial pathogenesis is well established but whether this system is involved in multicellular processes, such as bacterial biofilm forma...
Membrane vesicles from secretion buffer and infested rat serum were purified on sucrose gradient and electron microscopy pictures have shown 50- to 100-nm vesicles budding from the coated plasma membrane. Mass spectrometry confirmed the presence of Trypanosoma proteins in these microvesicles, showing ...
Lipidic cubic phases (LCP) are mainly formed by energetically mixing the chosen lipid with an aqueous buffer (the protein–detergent complex) at certain ratio and temperature. The cubic phase is formed when the matrix becomes a transparent and non-birefringent gel-like material. Structurally, ...
(biological triplicates) were resuspended in urea lysis buffer [8 M urea, 50 mM Tris-HCl (pH 8.0), and 1 mM sodium orthonovanadate supplemented with Complete Ultra tablets and Phosstop Phosphatase Inhibitor Cocktail tablets] and lysed with a cell disruptor (One Shot model; Constant System ...