2000. Protein-bound D-amino acids, and to a lesser extent lysinoalanine, decrease true ileal protein digestibility in minipigs as determined with 15N-labeling. J Nutr 130:2026-2031.de Vrese, M., R. Frik, N. Roos, & H. Hagemeister. 2000. Protein-bound D-amino acids, and to a ...
An aliquot (0.1 ml) was taken from the reaction mixture, and the amount of amino acids was determined by amino acid analysis as described in Experimental Procedures. MAbs directed to protein-bound HHE There is increasing evidence that aldehydes endogenously generated during the degradation process ...
When the addition of all amino acids is finished, the tRNA bound to the ribosomal P site has the complete polypeptide chain attached to its 3′ end. The open reading frame of the mRNA used for protein synthesis spans from the initial AUG codon to the completion signal (a UAA, UAG, or ...
The three-dimensional structure used to calculate the contact energies of apoMb was determined as follows: the all-atom molecular dynamics simulations of apoMb with heme removed from the heme-bound structure were performed using GROMACS 2021.2 with ff14SB for 1 μs with explicit solvents, as onl...
The fraction of the protein bound to the liposomes can be calculated as described in the Materials and Methods. While La alone did not pellet along with PS-containing liposomes in the absence of Ca2+, both La and Anx A5 were enriched with liposomes in response to Ca2+ and La’s ...
in the protein. Van der Waals forces and hydrophobic interactions also participate in the binding of the dye by protein. The number of Coomassie dye ligands bound to each protein molecule is approximately proportional to the number of positive charges found on the protein. Free amino acids, pept...
46The molecules created by hydrolysis should be too small to cross-link two IgE antibodies bound to the surface of mast cells. Failure to cross-link IgE antibodies prevents mast cell degranulation, thereby preventing an IgE-mediated (type I) hypersensitivity.47However, hydrolyzed proteins will not...
Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor. Nature 581, 215–220 (2020). Article PubMed CAS Google Scholar Ueda, T. et al. Enhanced suppression of a protein–protein interaction in cells using small-molecule covalent inhibitors based on an N-acyl...
Proteins bound to the column were eluted with a linear gradient of 0-1.0 M imidazole in 20 mM Tris-HCl (pH 8.0) containing 300 mM NaCl. Peak fractions were diluted ten-fold with 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM EDTA and loaded onto an Econo-Pac high-Q cartridge (Bio...
Even more interesting is the observation that granule-bound starch synthase (GBS1), a protein embedded in the starch matrix ([18, 19]) has protein levels which fluctuate diurnally [12] in the same manner as At5g39790. GBS1 does not have a carbohydrate-binding domain, and based on ...