Also with low amounts all the weights are clear! Applications: Western Blot FH Grace (Verified Customer) (01-15-2024) Empty heading Works perfectly and no issues with transferring onto the PVDF membrane for Western Blot Applications: Western Blot FH Tsimafei (Verified Customer) (12-11-2023)...
e Bone resorption in osteoclasts treated with 40 nM recombinant La 1-375 normalized to the control after adding the same amount of PBS. (n = 4) (P = 0.03) b–e Statistical significance was assessed via one-tailed paired t-test. Data are presented as mean values + /- ...
The most common way to quantify adsorption is by means of the adsorption isotherm, where at constant temperature, the amount of molecules adsorbed is plotted against the steady-state concentration of the same molecules in bulk solution. Adsorption isotherms provide a convenient method for determining ...
The amount of S-palmitoylated proteins is large and diverse; some of them are synthesized on soluble ribosomes but others are transmembrane-spanning proteins. Some of these proteins are modified sequentially with different lipids, but others are exclusively S-palmitoylated. In the past few years,...
Immunoblots of lgt-mutants demonstrated no changes in the molecular weight of CbpL and it must be further noted that the protein amount was not substantially altered compared to isogenic wild-type pneumococci (Fig. 1C). To demonstrate the abundance of CbpL on the pneumococcal surface and to...
For proteins that are difficult to elute from the gel such as large molecular weight proteins, a small amount of SDS may be added to the transfer buffer to improve transfer. We recommend pre-equilibrating the gel in 2X Transfer buffer (without methanol) containing 0.02-0.04% SDS for 10 ...
Equal amounts of total protein were loaded on 10% SDS-PAGE gel and transferred to nitrocellulose membranes that were further blocked with 5% skim milk and incubated overnight with primary antibodies toward GFP (cat. ab6877, Abcam), Ku70 (cat. sc-5309, Santa Cruz Biotechnology), β-Tubulin ...
Leafy greens: Depending on the types of greens you use, you can vary the amount of protein in this recipe. If you use kale, it’s got about 8 grams of protein in the 3 cups that go into this recipe. If you use spinach, you’ll get closer to 3 grams in those 3 cups. ...
Osmotic stress resuls in an alteration of availability of bulk water for (de)hydration reactions because of a change of the amount of bound and vicinal water around solutes (Douzou, 1994). Because (de)hydration reactions have been suggested to contribute substantially to the energetics of conforma...
Our experience with sample preparation has shown that there is a variation in the concentration of the necessary amount of sample required to achieve similar particle numbers in frozen grids when using the Vitrobot, TED, and chameleon. There have been previous studies which have shown that the blo...