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Our integrative analysis of chromatin maps and gene expression profiles revealed that the observed ASH1-FSH binding pattern at promoters is a hallmark of active genes. Inhibition of FSH-binding to chromatin resulted in global down-regulation of transcription. In addition, we found that genes ...
英文名称: rProtein A Sepharose FF 产地: 进口 品牌: HuicH 货号: RF1071 英文名称:rProtein A Sepharose FF 级别:BR基质:6%的交联琼脂糖凝胶配基:重组蛋白A 配基密度:6mg重组蛋白A/ml吸附载量:25mg人IgG/ml蛋白A残留:3-5ng蛋白A/mg IgG颗粒大小:50-160μm*流速:300cm/hpH范围:3~10性状(以下...
we used X-ray crystallography to obtain the structure of 14-3-3ε in complex with the GATAD1 phosphopeptide at 3.1 Å resolution (see Supplementary Table1for the complete data statistics). The two 14-3-3ε proteins in the asymmetric unit (ASU) of the crystals formed a two-fold symme...
Sonicated chromatin was centrifuged at 16,000g for 5 min and then incubated overnight at 4 °C with 1–2 μg antibody against p53 (DO-1, anti-human p53 monoclonal antibody) or normal mouse IgG in the presence of protein A/G-Sepharose beads (Invitrogen). Precipitated chromatin was...
实用案例二: Seebio rProtein G SeePUR重组蛋白G亲和层析预装柱在人血清IgG纯化中的应用 美国某知名品牌的rProtein G Sepharose 4B亲和层析柱对人血清进行纯化(图1,Lane 4~8为IgG洗脱管),其对人IgG的*结合量为约34 mg/mL Medium (其说明书称*结合量>25 mg/mL Medium); 使用西宝生物rProtein G SeePUR...
The digested cross-linked mitochondria and SV peptides were enriched by dibenzocyclooctyne (DBCO)-coupled sepharose beads. Briefly, the mitochondrial or SV peptides were resuspended to 3 mg/ml in PBS and were then added to the prewashed DBCO beads for incubation overnight at room temperature ...
and the rest of the supernatants were incubated with indicated antibodies overnight at 4 °C and mixed with Protein G Sepharose beads (GE Healthcare, Milwaukee, WI, USA) for 2 h at 4 °C. Immunoprecipitates were washed four to six times with respective lysis buffer, boiled in prot...
Refolded TCRs were purified by diethylaminoethanol (DEAE) sepharose anion exchange, followed by Superdex-75 16/60 gel-filtration (GE Healthcare) and anion exchange Mono Q 10/100 GL (GE Healthcare). The molecular weight of soluble refolded TCRs was validated by liquid chromatography (LC) ...
Dengue virus (DENV) infection causes a spectrum of dengue diseases that have unclear underlying mechanisms. Nonstructural protein 1 (NS1) is a multifunctional protein of DENV that is involved in DENV infection and dengue pathogenesis. This study investigated the potential post-translational modification ...