众所周知 Santa Cruz 是世界著名的抗体生产商,提供了数以万计的抗体,包括不断推出的单克隆抗体之外,近期还发布了了一种替代传统抗小鼠二抗的新型“二抗”——Mouse IgG Binding Protein 。 Mouse IgG Binding Protein,即小鼠结合蛋白,与小鼠IgG轻链(IgGκ /IgGλ )特异性结合,可替代传统的山羊/兔抗小鼠二抗,用于...
天然蛋白A(Protein A)是一种发现于金黄色葡萄球菌的细胞壁表面蛋白,天然蛋白G(Protein G)是一种分离自G型或C型链球菌属的细胞表面蛋白,二者功能相似,主要通过与免疫球蛋白(Ig)的Fc区相互作用,可结合大多数哺乳动物的IgG。然而两者结合特异性上有所不同(详细见附录1,蛋白A,G对不同物种Ig的结合能力总表),如蛋...
众所周知,Santa Cruz 是世界著名的抗体生产商,提供了数以万计的抗体,包括不断推出的单克隆抗体之外,近期还发布了了一种替代传统抗小鼠二抗的新型“二抗”——Mouse IgG Binding Protein。 Mouse IgG Binding Protein,即小鼠结合蛋白,与小鼠IgG轻链(IgGκ /IgGλ )特异性结合,可替代传统的山羊/兔抗小鼠二抗,用于...
M.W. of protein~25kDa (Protein A) Antibody concentration≥0.6mg Protein A per ml beads Binding capacity≥ 0.7mg human IgG per ml beads SpecificityAntibodies from many different species, including mouse, human, rabbit, cow, goat and sheep ...
The association constants of PROSEP-A and protein A bound to sepharose for mouse IgGl were determined and values of 1.15 x 10 5 M −1 ) and 0.97 x 10 5 (M −1 ) were obtained respectively. The equilibration time for binding mouse IgG1 to PROSEP-A High Capacity was determined to ...
Binding strength to protein A discriminates three definitive types of mouse monoclonal IgG1. Each of these types of IgG1 elutes from protein A-Sepharose as a coherent peak representing the great majority of IgG1 protein. One type of IgG1 is not present to any significant extent in polyclonal...
同时,两者对于不同的免疫球蛋白亚类的结合能力有所不同。适当重组改造的Protein A、G与磁珠以一定的方式结合,可用于免疫沉淀或抗体的纯化。 Protein A+G磁珠适合于免疫沉淀所有Protein A磁珠和Protein G磁珠单独可以免疫沉淀的抗体,包括human IgG1、IgG2、IgG3、IgG4,mouse IgG1、IgG2a、IgG2b、IgG3,rat IgG1...
种属抗体类型Protein A/GProtein A HumanTotal IgG+++++ IgG1,IgG2+++++ IgG3+++++ IgG4+++++ IgM-- IgD-- IgA++ IgA1,IgA2++ IgE+++++ Fab-- ScFv-- MouseTotal IgG+++++ IgM-- IgG1++++ IgG2a+++++ IgG2b++++ IgG3+++++ RatTotal IgG...
IgG1 - + +++ IgA2 + - +++ IgG2a - ++++ +++ IgD - - +++ IgG2b - ++ +++ IgE ++ - +++ IgG2c ++ ++ +++ IgM + - +++ IgG3 + ++ NT Rabbit IgG +++ +++ + Mouse IgG ++ ++ +++ Cow IgG + +++ - IgG1 + ++++ ...
Protein A+G琼脂糖凝胶适合于免疫沉淀所有Protein A琼脂糖凝胶和Protein G琼脂糖凝胶单独可以免疫沉淀的抗体,包括human IgG1、IgG2、IgG3、IgG4,mouse IgG1、IgG2a、IgG2b、IgG3,rat IgG1、IgG2a、IgG2b、IgG2c,以及rabbit、goat等多克隆抗体。下表是碧云天Protein A、Protein G、Protein A/G琼脂糖凝胶产品与人...