Protein A/G MagBeads (Cat. No. L00277, 2 ml 25% slurry) are average 40 μm in siz e, superparamagnetic beads covalently coated with Protein A/G. The beads are supplied as 25% slurry in phosphate buffer saline containing 20% ethanol. The Protein A/G MagBeads are ready-to-use for ...
Protein A G MagBeads 技术手册说明书 Protein A/G MagBeads Cat. No. L00277 Technical Manual No. TM0249 Version 08212013 Index 1.Product Description 2. Instruction For Use 3.Troubleshooting 4. General Information 1.Product Description 1.1Intended Use GenScript Protein A/G MagBeads...
rProtein A Beads使用说明
Protein A/G-Plus Beads抗体纯化柱使用说明 【产品说明】 本产品使用Protein A与Protein G的抗体结合结构域融合后的重组蛋白和4%浓度的琼脂糖树脂偶联制成。由于采用了重组表达的Protein A/G,并去除了其白蛋白结合区,该基质能够特异性地结合多种免疫球蛋白的Fc区段,作为亲和树脂,可用来纯化不同来源的抗体和抗体亚...
Protein A G免疫沉淀磁珠使用说明书 Protein A/G免疫沉淀磁珠 Figure 1. General Protocol for Immunoprecipitation complex SDS-PAGE loading buffer Neutralize buffer Magnetic Beads antibody Magnetic Separator Remove supernatant Pipette Repeat 45 产品组分 产品参数:磁珠粒径100 nm,浓度10 mg/mL,结合量>400 ...
Beads分散在含有0.01%叠氮化钠的PBS缓冲液中,用于免疫沉淀(IP)以及免疫共沉淀(Co-IP)研究。2 ml包装内含有0.5 ml Beads,可进行100次常规的免疫沉淀试验。 【使用说明】 A.样品的制备: 1.对于100 mm培养皿中的贴壁细胞,吸除细胞培养液,使用预冷PBS洗涤一次后加入2毫升细胞裂解液裂解细胞。对于悬浮细胞,离心...
PureProteome ™ Protein A and Protein G Magnetic Beads Eliminate Variability. Maximize Recovery.Advantages High capacity: More than 6x the • binding capacity of competitive magnetic beads.Bind 1.5-3.5 µg of rabbit IgG –per µL of suspension Consistent results: Total removal • of ...
可以用不同浓度的咪唑进行梯度洗脱,在样品及平衡缓冲液中添加0.5%吐温或Triton X-100,或5~50%的甘油,可以避免因为疏水相互作用导致非特异吸附,或使用钴离子螯合磁珠(BeaverbeadsTM IDA-Cobalt)。此外,要获得纯度较高的蛋白,需要对裂解、结合缓冲液、洗涤缓冲液、洗脱缓冲液的组分、pH、咪唑浓度等进行优化。若还是...
rProtein A G Beads 北京索莱宝科技有限公司 第1页共6页rProtein A/G Beads 货号:R8281 规格:2ml/5ml 保存:2-8℃指标 性能基质 4%琼脂糖微球配体 重组蛋白A/G 载量 >30mg 人lgG/ml 介质粒径(µm)45-165最大流速 0.1MPa,1bar pH 稳定范围 3-10储存缓冲液 20%乙醇储存温度 2°C-8°C ...
MCE_Protein_AG_Magnetic_Beads_Manual_cn