We find that ABP1 is genetically upstream of TIR1/AFBs; ABP1 knockdown leads to an enhanced degradation of AUX/IAA repressors, independently of its effects on endocytosis, through the SCF(TIR1/AFB) E3 ubiquitin ligase pathway. Combining positive and negative regulation of SCF ubiquitin-...
It has therefore been suggested that mutant p53 can act in a dominant-negative way, whereby such mutants would, in the heterozygous state, bind and inactivate the products from the wild-type TP53 gene, thus explaining the original observation that mutant p53 can contribute to cellular ...
In vitro, soluble α-Syn monomers spontaneously assembled into amyloid fibrils, as indicated by a binding assay with the amyloid-specific dye thioflavin-T (ThT) (Figure 1H, left) and by negative-stain electron microscopy (EM) (Figure 1I). GST-TRIM11, but not GST, suppressed de novoα-...
The resulting image now only contains 2|Escat|cos(Φj) projected along the positive and negative real and imaginary axes of a complex plane. By averaging the four images rotated all onto the positive real axis, the complex field Escat is recovered. For static measurements, particles were ...
Human antigen R (HuR) is an essential regulator of RNA metabolism, but its function in metabolism remains unclear. This study identifies HuR as a major repressor during adipogenesis. Knockdown and overexpression of HuR in primary adipocyte culture enhanc
Benz R, Schmid A, Hancock REW (1985) Ion selectivity of gramnegative bacterial pores. J Bacteriol 162:722–727 Google Scholar Berg DE, Berg CM (1983) The prokaryotic transposable element Tn5. Biotechnology 1:417–435 Google Scholar Beringer JE, Beynon JL, Buchanan-Wollaston AV, Johnston AW...
Members of the genusRickettsiaare obligate intracellular, Gram-negative, bacteria. These bacteria are surrounded by a crystalline proteic layer [1], also referred to as S-layer, which represents 10 to 15 % of their total protein mass [2] and is made of immunodominant surface protein antigens...
(1:50, #8480, Cell Signaling) at 4 °C overnight. Rabbit (DA1E) mAb IgG XP Isotype Control (1:50, #3900, Cell Signaling) was used as a negative control. Then, protein/DNA complexes were eluted and free DNA was purified for the following qRT-PCR assay. Promoter primers for ...
A negative correlation between the endogenous proteolytic activity in leaves adapted to progressive drought and the presence of BnD22 was established. The antiproteolytic activity of the purified mature BnD22 was assayed both on endogenous leaf proteases and purified serine proteases. A low level of...
These results suggest that Gpbl is a negative regulator of conjugation and sporulation that apparently works upstream of Ras1 function in S. pombe . The possible relationship of Gpbl to two previously identified, putative G α proteins of S. pombe is discussed. 展开 关键词: Gβ protein ...