The mechanisms involved in programmed or damage-induced removal of mitochondria by mitophagy remains elusive. Here, we have screened for regulators of PRKN-independent mitophagy using an siRNA library targeting 197 proteins containing lipid interacting d
DNA–protein crosslinks (DPCs) induced by aldehydes interfere with replication and transcription. Hereditary deficiencies in DPC repair and aldehyde clearance processes cause progeria, including Ruijs–Aalfs syndrome (RJALS) and AMeD syndrome (AMeDS) in
This suggests that down-regulation of Lpr6 (or Lnc2) alone is not sufficient to have a significant influence on the overall life span, which is plausible considering the complexity of aging. However, its reduced expression still has a positive and reproducible effect on the survival rate of C...
Finally, we note that nearly all studied IDPs (including those in this work) are excised from full-length proteins, in which they would be tethered to folded domains. The importance of IDP:folded domain interactions has already been pointed out in several recent studies36,37. Our results poi...
Both, NF-M and MAP(RA4) proteins, showed a dynamic pattern of expression coincident with the progression of retinal cell differentiation. Both proteins were coexpressed spatially and temporally in postmitotic neuronal precursors throughout development. Expression of both proteins was seen in ganglion ...
Before analysis of citrullinated proteins, false-positive peptides with citrullinated arginine in the C-terminal were manually excluded from the data. A citrullinated peptide was included in the analysis if it belonged to a protein present in at least 2 out of 3 individuals. Similarly, a peptide...
In other words these regulatory sequence elements are avoided and hence underrepresented across a single genome or across particular types of sequences (e.g., those coding for proteins) in a single genome. However, they are present in orthologous genes from multiple related organisms. Here we ...
mutations inNPM1result in mislocalization of NPM1 mutant proteins to the cytoplasm (NPM1c+). NPM1c+acts as a dominant negative mutant for NPM1 function and has a gain-of-function role in pathogenesis of the AML phenotype [196,197]. Although there are many different strategies in targeting...
Intrinsically disordered proteins and protein regions (IDPs) are prevalent in all proteomes and are essential to cellular function. Unlike folded proteins, IDPs exist in an ensemble of dissimilar conformations. Despite this structural plasticity, intramo
Samples were analysed in both positive and negative ion modes with a resolution of Rm/z = 200 = 280,000 for MS and Rm/z = 200 = 17,500 for MS/MS experiments, in a single acquisition. MS/MS was triggered by an inclusion list encompassing corresponding MS mass ranges...