Cofactor(prosthetic group) is a part of holoenzyme. What is Apoenzyme example? Apoenzyme is the protein part of an enzyme. The non-protein part cofactor together with the protein part apoenzyme forms a holoenzyme. ... For example,coenzyme NADHand the pyruvate substrate should be added to the ...
In enzymatic catalysis the prosthetic group is usually referred to as a coenzyme. Coenzymes are firmly bonded to the protein part of a biocatalyst by an apoenzyme and remain affixed to the protein molecule for the duration of the catalysis; examples of coenzymes include lipoic acid, riboflavin,...
Horseradish peroxidase may be split into heme and the apoenzyme, and a synthetic group, 1-anilino-8-naphthalenesulfonic acid, may take the place of heme in the protein. In this work it is shown that heme affords a strong protective effect against ultraviolet inactivation as well as against ...
aCoenzyme linked to apoenzymes not very tight and can be aparted from holoenzyme by dialysing, while prosthetic group linked tightly and can not be separated that way. 与apoenzymes不非常紧紧连接的辅酵素和可以是从holoenzyme aparted通过渗析,而方式紧紧连接的义肢小组并且不可能被分离。[translate]...
辅酶离开 apoenzyme 作为催化反应、 假肢 groupcan 不偏离全 翻译结果4复制译文编辑译文朗读译文返回顶部 Coenzyme离开apoenzyme作为推动一种反应,修复groupcan不背离holoenzyme 翻译结果5复制译文编辑译文朗读译文返回顶部 辅酵素事假apoenzyme作为摧化反应,义肢groupcan从holoenzyme不离去...
Preferred apoenzymes for use in the assay are apoglucose oxidase and apoperoxidase. The assay offers the advantages of colorimetric read-out and of being readily adaptable to automated techniques. Also disclosed are prosthetic group- labeled and enzyme- labeled conjugates for use in the assay, as...
The enzyme contained two mol FAD per mol enzyme (one covalently-bound and one noncovalently-bound; mol. wt., 174,000). The “semiapoenzyme”, which contains the covalently-bound FAD alone, was prepared by the acid-ammonium sulfate treatment. The semiapoenzyme had practically no activity for ...
Prosthetic group labeled enzyme immunoassay (PGLEIA) is a separation-free (homogeneous) enzyme mediated immunoassay. The assay depends on: (1) the ability of a stable covalent ligand-prosthetic group conjugate to activate an apoenzyme which by itself is enzymatically inactive; and (2) the ability...
The indicator is the prosthetic group residue alone or a holoenzymatic residue comprising the prosthetic group residue in combination with an apoenzyme in the form of a holoenzymatic complex. Application to the field of medical analysis.HORNBY WILLIAM E...
PURPOSE:To efficiently obtain an apoenzyme of an oxidoreductase containing pyrroloquinolinequinone (PQQ) as a prosthetic group, by treating the oxidoreductase containing the PQQ as a prosthetic group with a chelating agent. CONSTITUTION:An enzyme, containing pyrroloquinolinequinone (PQQ) and present in ...