RNA was gained from all cells holding one of the five shRNA constructs. A qPCR (OCT-1 and β-actin as housekeeping gene) was established to examine the efficacy of the OCT-1 knockdown (for primer sequences see Table 2). The cells with the most promising clone were chosen for an ...
(I) Active promoters exhibit more looping with distal CTCF sites than silent promoters. Genes were separated to active genes (RPKM ≥ 3) and silent genes (RPKM ≤ 3). The number of CTCF binding sites (>5 kb from TSS), which interact with the promoters, is plotted per promoter on y ax...
Plants of non-transformed control, transgenic rd29A:LeNCED1 lines 8F, 7D, 3D, and CaMV-35S:LeNCED1-12F line were grown in UC mix soils in greenhouse for 6 weeks under non- stress conditions and were subjected to drought treatment by with- holding irrigation for 3 days. Relative ...
Reardon W, Mullen AM, Sweeney T, Hamill RM: Association of polymorphisms in candidate genes with colour, water-holding capacity, and composition traits in bovine M. longissimus and M. semimembranosus. Meat Sci. 2010, 86: 270-275. 10.1016/j.meatsci.2010.04.013. Article CAS PubMed Google ...
Although relatively rare (1% of all malignancies in the US), the incidence of thyroid cancer more than doubled between 1984 and 2004 in the US. Between 1995 and 2004, thyroid cancer was the third fastest growing cancer diagnosis, behind only peritoneum, omentum, and mesentery cancers and “...
other, holding the fibers together at these points. The heat from the fibers vaporizes most of the water in the binder. The fiberglass binder should be flexible so that the fiberglass product can be compressed for packaging and shipping and later recover to its full vertical dimension when ...
In each experiment, catalysts (400 mg) were activated in H2 (30 mL min−1) (Airgas, San Antonio, TX, USA) by ramping (1 °C min−1) to 400 °C and holding for 1 h. Catalysts were then cooled to 50 °C in flowing H2 and were held at that temperature for 1 h. Argon (...
The PCR conditions were as follows; 94 °C for 3 min followed by 30 cycles of 94 °C for 45 s, 58 °C for 30 s, and 72 °C for 45 s, followed by a final extension at 72 °C for 5 min while the final holding temperature was 4 °C. The primer sets were as follows: (i)...
Albit also used by large agricultural holding Linas Agro Group AB (Lithuania – Denmark), RWA Raiffeisen Agro d.o.o. (Austria –Croatia). A leading agronomist on plant protection Martin Solorzano, who has been applying Albit on roses for many year, wrote the following: ‘Albit is a ...
Membrane capacitance was calculated as the integral of the transient current in response to a brief hyperpolarizing pulse from −80 mV (holding potential) to −90 mV. Transfection of C2C12 Cell with Antisense Oligonucleotides Sense and antisense oligonucleotides were synthesized and phosphorothioated ...