We have attempted to reactivate chromosome activity in dividing, permeabilized animal cells with the aim of analysing the physiology of chromosome movements, particularly during prometaphase. We achieved reactivation on numerous occasions, but it was limited in extent and unreliable in that many cells ...
To induce RS and arrest cells in the G2 phase • 3.1.1: (day 1, seed cells): Exponentially growing cells are seeded into one T75 flask at 30%confluencywith 10mL of cell culture medium. • 3.1.2: (day 2, treatment with or without APH and RO3306): To enrichprometaphasecells that...
A cell in which centrosomes reside at one side (i) or opposite sides (ii) of the nucleus at NEBD is shown. Scale bar: 5 μm. (B) Comparison between the prometaphase rosette (left) and monopolar spindle (right). HeLa cells expressing EGFP–α-tubulin (green), EGFP–CENP-A (green)...
Cell Division 19.1.3 Metaphase Metaphase begins when the chromosomes line up in a plane midway between the two poles (Walker, 1907; Bajer, 1958b; see Figs. 19.6 and 19.7). The formation of a metaphase plate is not essential for mitosis, and in cells, particularly those of the fungi, th...