(Dallas, TX, USA). Densitometry was performed using ImageJ software (NIH, Bethesda, MD, USA). Immunohistochemistry Immunohistochemistry was performed on formalin-fixed paraffin-embedded human breast tissues (normal, DCIS, carcinoma). Tissues were ethically obtained from the Queen Elizabeth Hospital and...
Next, by use of semi-dry transfer (15 V, 30 min), the proteins were transported onto a nitrocellulose membrane followed by 1 h incubation with 5% non-fat dried milk (Santa Cruz Biotechnology, Dallas, TX, USA) in TBS-T (20 mM Tris, 150 mM NaCl, 0.1% Tween-20, pH 7.6). The ...
DH/POX silencing in cell clones was checked by Western immunoblotting using an anti-PRODH/POX antibody (Santa Cruz, Dallas, TX, USA). The level of PRODH/POX knockdown is presented in (Supplementary Material Figure S1). The PRODH/POX knock-out C32 cells defined as C32POX−cells were ...
FoxO1, POX, ATGL or scramble siRNAs (Santa Cruz Biotechnology, Dallas, TX, USA) were transfected by using DeliverX Plus kit (Affymetrix, Santa Clara, CA, USA). Pc-DNA3.1 plasmid (Life Technologies) containing SOD2 cDNA or PcDNA3.1 empty vector was transfected by using Turbofect Transfection ...
(Dallas, TX); hnRNPM (A500–011A, RRID:AB_11125542) from Bethyl Laboratories (Montgomery, TX); horseradish peroxidase (HRP)-conjugated FLAG (A8592, RRID:AB_439702) from Sigma-Aldrich (St. Louis, MO); HA (11867423001, RRID:AB_390918) and HRP-conjugated HA (12013819001, RRID:AB_390917)...
We used 1 μL of 1 μg/mL Polybrene per mL of media (Santa Cruz #SC-134220, Dallas TX) to facilitate the transduction. The media was changed at 4 h post transduction. The virus-infected cells were then expanded, and frozen stocks were made for future differentiation. Ngn2 and rTTA ...
(Dallas, TX). Rabbit anti-α- SMA antibody (ab5694) was purchased from Abcam (Cambridge, United Kingdom). Biotinylated-ILB4 was purchased from Vector Laboratories (Burlingame, CA). Alexa 488 Streptavidin, Alexa ScieNtiFic REPOrTS | 7:43057 | DOI: 10.1038/srep43057 9 www.nature.com/...