FRET analyses were done with a monochromator-assisted digital epifluorescence video imaging setup (TILL-Photonics, Gräfelfing, Germany) equipped with a motorized emission filter wheel (Lambda 10/2, Sutter Instrument Co.). Determination of FRET efficiencies was performed as described earlier (27). ...
[34]. Fresh leaves near the roots were selected, 0.1 g weighed, and added to 1 mL PRO Lysis buffer to homogenize. After boiling in water for 10 min, samples were filtered with filter paper or gauze, and the filtrate is the proline extract. Set the proline standard sample gradient, 10...
Dried samples were crushed into fine powder with liquid nitrogen and homogenized with aqueous sulphosalicylic acid (20 mL, 3% w/v), and the homogenate filtered through Whatman # 2 filter paper. A total of 2 mL of the filtrate reacted with acid-ninhydrin solution (2 mL) and glacial acetic...
(ThermoFisher Scientific, Server version 2.15.3, TIA version 5.0) operated at an acceleration voltage of 300 kV and equipped with an extra-bright field emission gun, a BioQuantum post-column energy filter (Gatan), and a K3 direct electron detector (Gatan, Digital Micrograph version 3.32....
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Device finds signal outside the set filter range. – Pre-amplifier disconnected. – Loading a new amplifier software version or data into the device. No other commands possible at this point. Uploading the device data. No other commands possible at this point. Positive zero return active. ...
Iris lactea var. chinensis, a perennial herbaceous species, is widely distributed and has good drought tolerance traits. However, there is little information in public databases concerning this herb, so it is difficult to understand the mechanism underly
The seeds were dried under laminar flow air, and uniform seeds showing radicle emergence were selected and germinated in sterile Petri dishes layered with filter paper containing 4 mL of various treatment solutions. 4.2. Chemicals and Treatments Hematin (Ht) (cat # H3281, Sigma-Aldrich, Saint ...