The significant DEG threshold of nominal p value < 0.05 instead of FDR adjusted p value < 0.05 was used in this discovery analysis to mitigate the low sample size. Candidate pathways and genes were validated in independent replication transcriptomic datasets (see below). We performed ...
transcript levels in gastrocnemius muscle tissue of an affected individual were similar to those seen in the gastrocnemius of an ethnically matched healthy control sample (data not shown). DISCUSSION We describe a novel form of autosomal recessive spastic paraplegia caused by a variant in KY encoding...
We ran CellBender (v.0.1.0) on the unfiltered count matrix of each sample produced by SEQC (v.2.7.0) with the following parameters: (1) set the expected number of cells as the number of cells loaded into each 10x Chromium lane per sample (typically 5,000–10,000 cells); (2) set...
No statistical analysis could be performed because of the small size of the sample (only one patient); however, all experiments were per- formed in triplicate and were repeated twice, with freshly extracted total RNA samples in the second experiment. Unfortunately, we were unable to confirm ...
Sample loading in square brackets ([]) denote volumes used for NaPTA precipitation prior to PK digestion. Figure S5. Western blot analysis of PrPres in frontal cortex (FC) of the case 2 compared to cortical samples of known non-CJD neurological control cases, a variably protease sensitive ...
After ultracentrifu- gation (100,000 g, 20 min, 25 °C), the pellets were washed with 0.5 mL sterile saline and solubilized in so- dium dodecyl sulfate (SDS)–sample buffer and, then, run on a 5–20% gradient polyacrylamide gel (SuperSep™ Ace; Wako, Osaka, Japan). Proteins were...
AACN Progressive Care Certified Nursing Sample Questions: 1. Which of the following statements is true regarding the QT interval and long-QT syndromes? A)Acquired-type long-QT syndrome may be caused by electrolyte abnormalities, especially hyperkalemia ...
Genetically modified plants are widely used in agriculture and increasingly in ecological research to enable the selective manipulation of plant traits in the field. Despite their broad usage, many aspects of unwanted transgene silencing throughout plant
active lesions contain more inflammatory inducible nitric oxide synthase (iNOS)-positive microglia and macrophages, which are potential sources of oxidative damage. Microglia and macrophages form borders around deep grey matter lesions, although to a lesser extent than white matter lesions [148]....
Before stating Theorem2.2, we need to know the upper bound of the wimpy variance (i.e., Theorem2.1). Then, Theorem2.2shows how to use thek-MCERA to compute the upper bound of the maximum deviation\(S({\mathscr {F}},S)\), using only sample-dependent quantities. ...