This solution was placed in a 12.5-ml Beckman centrifuge tube (Beckman Coulter, Fullerton, CA, USA) with a 5 to 30% sucrose gra- dient layered on top and then centrafuged at 39,000 rpm for 24 hours at 4°C in a Beckman SW-41 rotor. After the centrafuge, 600 μl fractions of ...
Ewe IGS (estrus n = 14; diestrus n = 14) were removed with a surgical blade and collected in a sterile tube with 1 mL of RPMI 1640 (Gibco-Brl). After disaggregation of the tissue with a surgical blade, samples of whole IGS were centrifuged at 190 g for 10 min. Then, they were ...