Proenkephalin (PENK) belongs to a family of three genes encoding peptides possessing opiate-like activity. Expression of the PENK gene is developmentally regulated in a tissue-specific manner. Widely expressed during embryogenesis in mitotic cells (K eshet et al. 1989), its expression is ...
Gene expression was confirmed by the presence of proenkephalin mRNA and proenkephalin-derived polypeptides in extracts of the SK-N-MC cells and also in the neuroblastoma SH-SY5Y cell line. In the latter cells, however, the expression was approximately 3 times lower, there was less processing ...
laevis, each of which codes for seven Met-enkephalin sequences but no Leu-enkephalin, indicating that Met-enkephalin preceded Leu-enkephalin in the evolution of the proenkephalin gene. The organization of the main exons of the toad genes is remarkably similar to that of the human gene and ...
BMSCPreproenkephalinTASAnalgesiaTransgenic therapy for central neuralgia faces the problems of low expression and weak targeting and affects superficial but not deep neurons. In this study, we generated a lentivirus vector with human preproenkephalin gene (hPPE) expression driven by the transcriptional ...
Enkephalins (leu- and met-enkephalin) arepentapeptidesbelonging to the endogenousopioid peptidesfamily (Noda et al., 1982). Both are products of theproenkephalingene. Theamino acid sequenceof Met-enkephalin is Tyr-Gly-Gly-Phe-Met and of Leu-enkephalin is Tyr-Gly-Gly-Phe-Leu. Both bind pref...
The effects of the pituitary adenylase cyclate-activating peptides (PACAP) 27 and 38 on proenkephalin (PENK) gene transcription were examined in PC12 (rat pheochromocytoma) cells using transient transfection assays. Both ligands stimulated PENK gene transcription in a dose-dependent manner, with an...
PENK/proenkephalin cDNA ORF Clone in Cloning Vector, Mouse 基本信息 基因 种属 Mouse NCBI 参考序列号 NM_001002927.2 参考序列ORF长度 807 bp 序列描述 Identical with the Gene Bank Ref. ID sequence. 描述 Full length Clone DNA of Mouse preproenkephalin. 质粒 载体 pGEM-T Vector 测序引物...
Antinociceptive Effect of Intrathecal Injection of Genetically Engineered Human Bone Marrow Stem Cells Expressing the Human Proenkephalin Gene in a Rat Model of Bone Cancer Pain 5-CTTGTGTTCCCTGTGTGCAGTG-3 (forward) 3-AGCAACCTCATTCTCCAAGTCA-5 (reverse) for PDyn mRNA 5-GAAGATGGATGAGCTTTACCCC-3...
We find that the general organization of the preproenkephalin gene is strikingly similar to that of the gene encoding the common precursor of corticotropin (ACTH) and β-lipotropin (β-LPH) 7–9 (alternatively designated preproopiomelanocortin), another multi-hormone precursor. Furthermore, the ...
(YGGFL). These are generated from aprecursor proteincalledproenkephalinby posttranslational proteolytic cleavage. In mammals,proenkephalincontains seven enkephalin sequences: six copies of Met-enkephalin and one copy of Leu-enkephalin. Thus, the activity of the proenkephalin gene is amplified via the...