R. (1997). Production and analysis of transgenic mice containing yeast artificial chromosomes. In "Genetic Engineering" (J. K. Setlow, Ed.), Vol. 19, pp. 235-255, Plenum, New York.Peterson KR. Production and an
Here we describe the production of cystic fibrosis transmembrane conductance regulator (CFTR), the product of the gene associated with cystic fibrosis, in the milk of transgenic mice. Mammary specific expression was achieved by placing the CFTR cDNA unde
Gordon JW, Ruddle FH (1983) Gene transfer into mouse embryos: production of transgenic mice by pronuclear injection. Methods Enzymol 101:411-433Gordon, J. W. and Ruddle, F. H. (1983) Gene transfer into mouse embryos: production of transgenic mice by pronuclear injection. Methods Enzymol. ...
Tumours producing regulatory peptides or amines common to the nervous and endocrine systems are called neuroendocrine tumours [1, 2]. They are composed of variably differentiated cells that share a number of morphologic markers normally expressed by neurons and endocrine cells [3, 4]. In laboratory...
Four lines of transgenic mice harboring a human insulin cDNA with expression driven by the goat β-casein gene promoter were generated. The expression level of human proinsulin in milk was as high as 8.1 g/L. The expression of the transgene was only detected in the mammary gland during ...
Production of human lactoferrin in the milk of transgenic mice and the generation of transgenic cows harbouring a transgene encoding human lactoferrin: H. de Boer, W. Eyestone, F. Pieper, R. Strijker, J. Nuijens, S. He Lee and P. Krimpenfort. Gene Pharming Europe B.V., Leiden, ...
Chimeric antigen receptor T cell (CAR-T) therapy has revolutionized the treatment approach for cancer, autoimmune disease, and heart disease. The integration of CAR into T cells is typically facilitated by retroviral or lentiviral vectors. However, the r
Objective The founder transgenic mice were generated to build a line of transgenic mice expressing human CD59 protein as an animal model for further study of xenograft rejection. Methods The transgene pEGFP-C1-OMT-CD59 containing human CD59 cDNA and green fluorescent protein gene (GFP) was construct...
TG: transgenic mice; WT: wild type. G GFP sequence in the offspring detected by Southern blot analysis. TG: transgenic mice; WT: wild type. H The methylation status of the DMRs of Peg10 and H19 genes in the mother (F0) and offspring (F1), as shown by bisulfite sequencing analysis. ...
Antosova Z, Mackova M, Kral V, Macek T: Therapeutic application of peptides and proteins: parenteral forever?. Trends Biotechnol. 2009, 27: 628-635. 10.1016/j.tibtech.2009.07.009. Google Scholar Shire SJ, Shahrokh Z, Liu J: Challenges in the development of high protein concentration for...