To determine in which process both DNA strands are transcribed, we need to analyze the options provided:1. PCR Technique: The Polymerase Chain Reaction (PCR) is a method used to amplify DNA. It does not involve transcription, a
were used with the chromosome ofC. subterminaleas a template to amplify a DNA fragment of the up- and down-stream region including N- and C-terminal sequence of the kamA gene, respectively. The sequence analysis with amplified DNA fragments was carried out following purification and resulted in...
The present invention is a modification of inverse PCR, where biotinylated inverse primer is used to amplify specific sequence using undigested genomic DNA. Following this, the sepcific PcR amplified fragments are enriched from the total pool by using streptavidin paramagnetic particles. Since only ...
What would happen if the sequence in the mRNA was not complimentary base-paired correctly from the sequence in the DNA? (Hint: there is a mutation in the sequence being used for protein synthesis.) What is the polymerase chain reaction (PCR)? How can this ...
1. A process of recovering oil, comprising (a) converting a starch-containing material into dextrins with an alpha-amylase; (b) saccharifying the dextrins using a carbohydrate source generating enzyme to form a sugar; (c) fermenting the sugar in a fermentation medium into a fermentation produc...
Current base- and prime-editing technologies lack efficient strategies to edit multiple genomic loci simultaneously, limiting their applications in complex genomics and polygenic diseases. Here, we describe drive-and-process (DAP) CRISPR array architectu
In the current study, the complete mitochondrial DNA of sixteen bird species from the family Ardeidae was sequenced and the evolution of mitochondrial gene rearrangements was investigated. The mitochondrial genomes were then used to review the phylogenies of these ardeid birds. Results The complete ...
The XcmI enzyme leaves a single base overhang that can be tailored to be a 5′T-overhang on both open ends of the backbone17. This results in a T-tailed vector after XcmI digestion and the approach is named by the inventor the "zero background" cloning. DNA fragments generated in ...
Suitable primers can be synthesised using the nucleotide sequence shown in SEQ ID NO.1 and these are then used to amplify by means of the polymerase chain reaction (PCR) thrE genes of various coryneform bacteria and strains. The person skilled in the art may find details of this in for ex...
Then, the IS compensates for this insufficient, increasing the number of macrophages and other phagocytic cells that lead to proinflammatory cytokines production in response to pathogenic microorganisms. These cytokines amplify the inflammatory response and induce a generalized inflammatory process in the ...