A process for DNA sequencing by the multi-primer method involving the use of oligonucleotide banks of hexamers, heptamers, octomers, or nonamers for the creation of the primer through the ligation of two or more oligomers following hybridization onto the primer site.Também publicado como EP...
摘要：A DNA sequencing process using specific stable isotopes associated with specific terminators for labels. The process includes a step of incorporating a stable isotope in at least one of the deoxynucleoside triphosphates and/or the dideoxynucleoside triphosphates such that a terminated strand has ...
The invention discloses a process for sequencing hydroxymethylation and methylation long-sequence tags and provides a method for detecting methylated modification and hydroxymethylation modification of nucleic acid. The method comprises the following steps: carrying out glycosylation modification and control ...
The present invention concerns obtaining a simple sequencing process for reallocating previously stored channels to the desired program setting number. A microprocessor receives a command, and within a time window determined by the command, reads previously programmed data concerning two selected program ...
deepTools addresses the challenge of handling the large amounts of data that are now routinely generated from DNA sequencing centers. deepTools contains useful modules to process the mapped reads data for multiple quality checks, creatingnormalized coverage filesin standard bedGraph and bigWig file forma...
Improved cycle sequencing of GC-rich templates by a combination of nucleotide analogs. A common problem in automated DNA sequencing when applying the Sanger chain termination method is ambiguous base calling caused by band compressions. Band ... M Motz,S Pääbo,C Kilger - 《Biotechniques》 被...
Characteristics of age-dependent genes in microglia across the adult lifespan To obtain deep insights into how microglia react during the aging process, we profiled microglial transcriptomes via bulk RNA sequencing (RNA-seq) on fluorescence-activated cell sorting (FACS)-isolated microglia (CD11b+ CD...
Cloning, sequencing, and expression of a Thermomonospora fusca protease gene in Streptomyces lividans. The major Thermomonospora fusca YX extracellular protease gene (tfpA) was cloned into Escherichia coli and Streptomyces lividans and was sequenced. The ope... Lao,D B,Wilson - 《Applied & Envi...
Experimental design and workflow of RNA-sequencing To develop a new anti-cancer therapy with the use of gallic acid, the first critical step is to understand the molecular mechanism governing this cell death process. RNA-sequencing is an important technology to understand the complexity of phenotypes...
Clones were expanded and were validated by western blotting and DNA sequencing. Mouse atrial cardiomyocytes HL-1 cells, obtained from the lab of Sigolene Meilhac (Institut Imagine/Université Paris Cite), were cultured on fibronectin (0.5%)/gelatin(0.02%) coated flasks in Claycomb medium (Sigma-...