Date Nov. 30, 1989.A process for DNA sequencing by the multi-primer method involving the use of oligonucleotide banks of hexamers, heptamers, octomers, or nonamers for the creation of the primer through the ligation of two or more oligomers following hybridization onto the primer site....
The invention discloses a process for sequencing hydroxymethylation and methylation long-sequence tags and provides a method for detecting methylated modification and hydroxymethylation modification of nucleic acid. The method comprises the following steps: carrying out glycosylation modification and control ...
Characteristics of age-dependent genes in microglia across the adult lifespan To obtain deep insights into how microglia react during the aging process, we profiled microglial transcriptomes via bulk RNA sequencing (RNA-seq) on fluorescence-activated cell sorting (FACS)-isolated microglia (CD11b+ CD...
deepTools addresses the challenge of handling the large amounts of data that are now routinely generated from DNA sequencing centers. deepTools contains useful modules to process the mapped reads data for multiple quality checks, creatingnormalized coverage filesin standard bedGraph and bigWig file forma...
Purification of mutacin III from group III Streptococcus mutans UA787 and genetic analyses of... Examines the cloning and sequencing of mutacin III biosynthesis locus gene from a group III strain of Streproccocus mutans (S. mutans). Isolation and purif... Fengxia,Qi,Ping,... - 《Applied &...
The genomic DNA extraction was carried out using the E.Z.N.A.® Power Soil ™ DNA Kit (Mo Bio Laboratories, USA) following the manufacturer's protocol. The bacteria 16s ribosomal RNA gene sequencing was amplified by the PCR system in the Illumina Miseq sequencing platform using universal...
Population Demographics ofT. cacao.aMaximum likelihood tree generated byTreeMixusing intergenic regions of whole-genome sequencing data from individuals belonging to each one of the 10 main genetic groups.bMaximum likelihood tree allowing for admixture, as generated byTreeMix, showing some of the most ...
amplifying HIV-1 subtype C env gp 120 and gag-protease genes by PCR form the viruses present in the peripheral blood cells of the infected individuals; sequencing two env genes and gag-protease gene; codon optimizing the sequenced genes cloning the codon optimized genes in plasmid vector.收藏...
To characterize the atlas of porcine spermatogenesis, we profiled the transcriptomes of ~ 16,966 testicular cells from a 150-day-old pig testis through single-cell RNA-sequencing (scRNA-seq). The scRNA-seq analysis identified spermatogonia, spermatocytes, spermatids and three somatic cell types ...
fingolimod is used as the HCl salt in the treatment of multiple sclerosis, and a very detailed patent provides three processes for its preparation that in... Turner,Keith - 《Organic Process Research & Development》 被引量: 0发表: 2010年 Mostly Natural DNA Sequencing by Synthesis The invention...