The development of biotherapeutics like antibodies comes with many challenges. Learn more about monoclonal antibody process development..
van Reis, Advances in technology and process development for industrial-scale monoclonal antibody purification, in: U. Gottschalk (Ed.), Process Scale Purification of Antibodies, John Wiley & Sons, New Jersey, 2008, pp. 203-221.N. Fontes, R. van Reis, Advances in technology and process ...
Monoclonal antibodies constitute a robust class of therapeutic proteins. Their stability, resistance to stress conditions and high solubility have allowed the successful development and commercialization of over 40 antibody-based drugs. Although mAbs enjoy a relatively high probability of success compared wit...
2007. Process analytics for purifica- tion of monoclonal antibodies. J Chromatogr B Analyt Technol Biomed Life Sci 848(1):79-87.Flatman S, Alam I, Gerard J, Mussa N. Process analytics for pu- rification of monoclonal antibodies. J Chromatogr B. 2007;848:79-87....
End-to-end continuous bioprocessing: impact on facility design, cost of goods, and cost of development for monoclonal antibodies Biotechnol. Bioeng., 118 (9) (2021), pp. 3468-3485, 10.1002/bit.27774 View in ScopusGoogle Scholar [15] V. Warikoo, R. Godawat, K. Brower, S. Jain, D. ...
Monoclonal Antibodies Solutions for mAb development and manufacturing Monoclonal antibody development and manufacturing are highly specialized processes, demanding equally specialized equipment. We will discuss some essential tools and systems in this article. ...
Recombinant monoclonal antibodies are predominantly produced in mammalian cell culture bioprocesses. Post-translational modifications affect the micro-heterogeneity of the product and thereby influence important quality attributes, such as stability, solubility, pharmacodynamics and pharmacokinetics. The analysis of...
Cells (5 × 103) were either centrifuged (Cytospin™) onto microscope slides or cultured for several hours on coverslips, fixed (4% PFA), permeabilized (1% Triton™ X-100/PBS, 10 min), and stained for ΔNp63 or PAX6, using antibodies as specified in Table S2 (Additional file...
hybridomas separately producing a monoclonal antibody, which specifically reacts with a Fas ligand present on a cell surface, a method of detecting a Fas ligand in a solution, and a kit for use in detecting a Fas ligand, comprising plurality of monoclonal antibodies against Fas ligand in combin...
it is indicated that hamster polyoma virus major capsid protein forms virus-like particles that might be used in medicine and veterinary science as tools for gene therapy, vaccine development and diagnostics. However, the use of these recombinant proteins to generate monoclonal antibodies of desired ...