For different experimental requirements, TIANGEN has developed a series of real-time PCR products, including the SuperReal product series with dual enzyme system, the fast reaction FastFire series, the Talent qPCR PreMix for complex templates, as well as the FastKing One Step RT-qPCR kits for fa...
Probe-based real-time PCRTaqManNeospora caninum is an apicomplexan protozoan parasite that is a leading cause of abortion in cattle. Detection of parasite-specific DNA by PCR is a highly sensitive method for identifying the presence of N. caninum in a variety of tissues. We developed and ...
Add 2.33 µl of the RT reagent mix each into a 0.2 ml PCR tube/plate. Vortex the RT primer tubes to mix, then centrifuge briefly at 10,000 x g for 10 sec. Add 1 µl of the miRNA specific RT primer to their respective PCR tubes or plate. Add 1.67 µl of the diluted RNA...
A 1.5hour DNA probe-melt genotyping real-time PCR reaction for Xylella fastidiosa. ? 9 loci genotyped by adjacent-binding DNA probes. ? $0.17 USD of reagents are used per PCR reaction. ? Works on DNA from environmentally-isolated samples (no culturing required). ? Robust even when ...
Evaluation of a Probe-Based PCR-ELISA System for Simultaneous Semi Quantitative Detection and Genotyping of Human Cytomegalovirus (HCMV) Infection in Clinical... M Talkhabifard,N Javid,A Moradi,... - 《Open Microbiol J》 被引量: 2发表: 2017年 Detection of PCR-amplified fungal DNA by using...
Universal probe-based and intermediate primer-triggered qPCR (UPIP-qPCR) is a two-step process. The first step, a standard PCR reaction, takes c. 30 mins. The second step, the qPCR reaction which uses the product of the first step, takes c. 60 mins, to obtain the corresponding fluoresce...
o.cena.com.cn|基于9个网页 2. 基于探测的方法 对于单播的方式,有两种速率控制方法,基于探测的方法(probe-based)和基于模型的方法(model-based)。基于探测的方法通过 … www.cnii.com.cn|基于2个网页 例句
Type: Realtime-PCR (Probe-based qPCR) Catalog Number: EZ3304-R21 / EZ3304-R22 / EZ3304-R23 Pack Size: 1 ml / 5 ml / 25 ml Price: 150 USD / 425 USD / 1750 USD Promotion: 120 USD / 340 USD / 1400 USD (920 Customers Bought) MANUAL Description This kit is a dedicated premixed...
Separate quantification of three classes of tfdA genes was performed using TaqMan quantitative real-time PCR for 13 different soils subsequent to mineralization of three phenoxy acids. Class III tfdA genes were found to be involved in mineralization more often than class I and II tfdA genes.关键...
The LNA probe-based real-time PCR assay was developed in a two-tube format with three LNA probes and one internal amplification control probe in each tube. The assay showed excellent specificity toM.tuberculosiswith or without RFP resistance by evaluating 12 strains of common non-tuberculosis ...