For different experimental requirements, TIANGEN has developed a series of real-time PCR products, including the SuperReal product series with dual enzyme system, the fast reaction FastFire series, the Talent qPCR PreMix for complex templates, as well as the FastKing One Step RT-qPCR kits for fa...
The objective of this study was to develop multiplex real-time quantitative PCR assays using hydrolysis probes targeting the 尾-tubulin gene to detect, distinguish, and quantify fungi associated with the polyphagous shot hole borer (PSHB; Euwallacea whitfordiodendrus, Fusarium euwallaceae, Graphium...
A 1.5hour DNA probe-melt genotyping real-time PCR reaction for Xylella fastidiosa. ? 9 loci genotyped by adjacent-binding DNA probes. ? $0.17 USD of reagents are used per PCR reaction. ? Works on DNA from environmentally-isolated samples (no culturing required). ? Robust even when ...
et al. A duplex TaqMan probe-based real-time PCR method for rapid detection and differentiation of the classical and VB strains of human immunodeficiency virus. Sci. China Life Sci. 67, 208–210 (2024). https://doi.org/10.1007/s11427-022-2337-5 Download citation Received17 January 2023 ...
Separate quantification of three classes of tfdA genes was performed using TaqMan quantitative real-time PCR for 13 different soils subsequent to mineralization of three phenoxy acids. Class III tfdA genes were found to be involved in mineralization more often than class I and II tfdA genes.关键...
Git, A., et al.Systematic comparison of microarray profiling, real-time PCR, and next-generation sequencing technologies for measuring differential microRNA expression.RNA.16, 991-1006 (2010). Benes, V., Castoldi, M.Expression profiling of microRNA using real-time quantitative PCR, how to use...
o.cena.com.cn|基于9个网页 2. 基于探测的方法 对于单播的方式,有两种速率控制方法,基于探测的方法(probe-based)和基于模型的方法(model-based)。基于探测的方法通过 … www.cnii.com.cn|基于2个网页 例句
Amplification using quantitative PCR (qPCR) is measured by detecting changes in a fluorescent signal. Fluorescently labeled probes are frequently used as the fluorescent reporter in qPCR. The GoTaq(R) Probe qPCR and RT-qPCR Systems are ready-to-use 2X master mixes optimized for probe-based qPCR...
Development of a Scorpion probe-based real-time PCR for the sensitive quantification of Bacteroides sp. ribosomal DNA from human and cattle origin and evaluation in spring water matricesDevelopment of a Scorpion probe-based real-time PCR for the sensitive quantification of Bacteroides sp. ribosomal DN...
(2014) Development of novel AllGlo-probe-based one-step multiplex qRT-PCR assay for rapid identification of avian influenza virus H7N9. Archives of Virology 159 , 1707-1713 /Zhang Y, Mao H, Yan J, Wang X, Zhang L, Guus K, Li H, Li Z, Chen Y, Gong L, Chen Z, Xia S: ...