PeT.cT2ti6hn5egPsetchsoeymnsaepmragreiecfdleuftofoerctohtpsehotobersmee.rpveerdatiunrefluefofreecstcseonbcseeqrvueedncfohrinVgPmRiΔgCh_t In this study we demonstrated that insertion of proline residues into loops contributed significantly to the tsutarbeitlhitaytoefnVfoPrcReΔsCpvrea-reiaxnist...
The short fragments of mRNA were used to transcribe first-strand cDNA using reverse-transcriptase (Invitrogen) and random hexamer-primers. The synthesis of second-strand cDNA was accomplished using DNA polymerase I (New England BioLabs, Ipswich, MA, USA) and RNase H (Invitrogen). Subsequently, ...
The RAPD-PCR was performed by adding 1× Taq DNA buffer, 1.5 mM of MgCL2, 0.2 mM of each deoxynucleotide, 1 µM of each primer, 2 ng of genomic DNA and 2U of Taq DNA polymerase enzyme, in a final volume of 25 µL. All reactions were assembled in duplicate and the amplification...