Further, we show that for Nurr1, phRG-B (a promoterless reporter plasmid containing synthetic Renilla luciferase gene) is a better control reporter vector than HSV-TK containing vectors. Finally, we noted the lack of effect of Nurr1 protein on the Fas Ligand promoter-driven transcription....
pRL 载 体包含一个编码海肾萤光素酶(Rluc) 的cDNA, 这个cDNA 从珊瑚虫类腔肠动物海肾(Renilla reniformis) 中克隆而来。目前 有四个不同的启动子构造。其中HSV- 胸腺嘧啶核苷激酶启动子(pRL-TK) 相对较弱, 更适用于海肾萤光素酶报告基因载体 的中性组成型表达。早期SV40 增强子/ 启动子区域(pRL-SV40) 和...
Renilla luciferase is a 36kDa monomeric protein that does not require post- translational modification for activity (2). Therefore, like firefly luciferase, the enzyme may function as a genetic reporter immediately following translation. For information about the use of this plasmid in conjunction with...
Further, we show that for Nurr1, phRG-B (a promoterless reporter plasmid containing synthetic Renilla luciferase gene) is a better control reporter vector than HSV-TK containing vectors. Finally, we noted the lack of effect of Nurr1 protein on the Fas Ligand promoter-driven transcription. ...
pRL 载 体包含一个编码海肾萤光素酶(Rluc) 的cDNA, 这个cDNA 从珊瑚虫类腔肠动物海肾(Renilla reniformis) 中克隆而来。目前 有四个不同的启动子构造。其中HSV- 胸腺嘧啶核苷激酶启动子(pRL-TK) 相对较弱, 更适用于海肾萤光素酶报告基因载体 的中性组成型表达。早期SV40 增强子/ 启动子区域(pRL-SV40) 和...
pRL-TK Vector base 1264 (T→C) to eliminate an internal BglII site, base 1807 (T→C) to eliminate internal BamHI site, base 1840 (C→T) to eliminate internal NarI, KasI, BanI and AcyI sites. Description The pRL Reporter Vectors contain a cDNA (Rluc) encoding Renilla luciferase, ...
Firefly luciferase activity was normalized to Renilla reniformis luciferase activity. 实验载体的构建:2.2kb的人的酪氨酸酶启动子/增强子区克隆到pGL3-Basic的萤光素酶的上游 E1500, E4030 Nonhomogeneous assay High level of luminescence Needs cell extract Using the Reporter Lysis Bufer (RLB), other assays...