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PP2A phosphatase activity was determined using Serine/Threonine Phosphatase Assay System by Promega (WI, USA) following the manufacturer’s instructions. Cells were lysed and subjected to Sepadex G-25 columns to eliminate cellular free phosphate, then protein concentrations were quantified. PP2A phosphata...
breve UCC2003-pAM5, and the Chromeleon software v. 6.70 (Dionex Corporation) was used for the integration and evaluation of the chromatograms obtained. Microarray data accession number The microarray data obtained in this study have been deposited in NCBI’s Gene Expression Omnibus database and ...
1. Protein phosphorylation plays a crucial role in regulating protein activity, turnover, and functions. Protein kinases and phosphatases are two opposite families of enzymes that regulate the dyna...
An SPSS software (Version 26, SPSS Inc., Chicago, IL, USA) was used for the statistical analysis. “p” values of <0.05 were considered statistically significant. 4. Results We prospectively screened 102 children from January 2023 to January 2024. Of these, 16 were excluded for not meeting...
Download: Download full-size image Figure 5. Enhancer activity within a region of the first intron of the human PRL-1gene. Transfections were performed in HepG2 cells. A, deletion analysis of intron 1 of the PRL-1 gene. The localization of deletions spanning intron 1 and the relative luci...
We found that the effect of TMZ alone on these cells after 72 h, was dose-dependent with an IC50 of 1 mM (Figure 4A) determined, with the GraphPad Prisme 6 software, by a nonlinear regression analyze using log (concentration) values. Interestingly, a combination of TMZ (0.5 mM) and ...
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Suppose you want to build your software in parallel (which is where Paul McKenney's series comes in). Well, you can't, because your entire build system is based on imperative code, and unless all the libraries you're using were written with that in mind...well, you're screwed. The ...
单击 SOFTWARE(软件). 下载 Instrument configuration 软件. 将下载的文件解压缩到本地计算机上的文件夹中. 使用下载的仪器配置定义系统,请参见 节 4.3.3 根据系统调整 UNICORN, 在第 页 51. 50 ÄKTA go 操作说明 29360951 AD 4.3.3 4 安装 4.3 软件安装 4.3.3 根据系统调整 UNICORN 根据系统调整 UNICORN...