Principles of PCR - Polymerase Chain Reactionamar nagesh
一步法檢測同時運用反轉錄酶和DNA聚合酶,將反轉錄和PCR步驟結合於同一個試管與緩衝液中。一步法RT-qPCR僅使用序列特異性性引子。在兩步法檢測中,反轉錄和PCR步驟在各自的試管中進行,具備不同的優化緩衝液、反應條件與引子啟動策略。 點擊圖片放...
Elimination of PCR duplicates in RNA-seq and small RNA-seq using unique molecular identifiers. BMC Genomics 19, 531 (2018). PubMed PubMed Central Google Scholar Benjamini, Y. & Speed, T. P. Summarizing and correcting the GC content bias in high-throughput sequencing. Nucleic Acids Res. ...
Detection of low concentration of genetically modified (GM) ingredients: While traditional PCR may give false negative results at low copy number, dPCR is able to directly calculate the gene copy number, and can accurately detect GM ingredients even if the content of GM ingredients is very low ...
The use of pesticides made it possible to increase yields, simplify cropping systems, and forego more complicated crop protection strategies. Over-reliance
The epicardium, the mesothelial envelope of the vertebrate heart, is the source of multiple cardiac cell lineages during embryonic development and provides signals that are essential to myocardial growth and repair. Here we generate self-organizing human pluripotent stem cell-derived epicardioids that ...
Larry E. Morrison.Basic Principles of Fluorescence and Energy Transfer Applied to Real-Time PCR[J]. Molecular Biotechnology .2010(2)Morrison L (2010) Basic principles of fluorescence and energy transfer applied to Real-Time PCR. Mol Biotechnol 44:168–176...
Currently, the most widely used nucleic acid detection methods in clinical practice include quantitative real-time PCR (qPCR), fluorescence in situ hybridization (FISH) and next-generation sequencing (NGS), which have reshaped the landscape of diagnosis by allowing massive molecular-level information ...
PCR, polymerase chain reaction; ELISA, enzyme-linked immunosorbent assay; PFGE, pulsed-field gel electrophoresis. Immunologic assays are also used to look for evidence that an agent-specific immune response has occurred in an exposed or potentially exposed individual. Serologic tests detect pathogen-...
During meiotic prophase, chromosomes organise into a series of chromatin loops emanating from a proteinaceous axis, but the mechanisms of assembly remain unclear. Here we use Saccharomyces cerevisiae to explore how this elaborate three-dimensional chromo