phase composition of multi-step linear gradient liquidchromatography is discussed with simultaneously considering effects of position of solute inside thecolumn and mobile phase composition on peak resolution and retention value, then a BASIC programbased on this principle is developed in IBM-PC computer...
phase composition of multi-step linear gradient liquidchromatography is discussed with simultaneously considering effects of position of solute inside thecolumn and mobile phase composition on peak resolution and retention value, then a BASIC programbased on this principle is developed in IBM-PC computer...
Use a 3nh spectrophotometer or colorimeter to measure the color of the sample under UV and UV-free light sources, and compare the data to separate the fluorescence effect. The emission spectrum characteristics of the Optical Brightening Agents (420-480 nm) will form a peak in the result, and...
Note that in the Figures 1-4 the spectra are normalized so that the peak maximum near 275 nm has been given the value 1. DCHA‐Iso 1,4 1,2 1 0,8 0,6 0,4 0,2 0 200 225 250 275 300 325 350 Wavelength (nm) Beermaster Iso octane Figure 1. Water based DCHA-Iso sample ...
2B). In the presence of PC/oleic acid, CT eluted from the column as a single peak corresponding exactly to the peak of radiolabeled [14C]oleic acid, and much earlier than the peak of 3H-labeled Triton X-100 present in the sample. No CT protein eluted in fractions between 26 to 29 ...
RP-HPLC separation of this fraction yielded a number of peaks, the major of which were found toxic. According to analytical HPLC and MALDI-MS, the predominant peak (∼10% of crude venom protein) was represented by a single component (>95% purity) with a molecular mass of 15,100 Da ...
but HPLC gave the best purification. The active principle as isolated by HPLC was quite pure because it gave very sharp single peak and also gave a single spot of approximate representative Rf value of 0.36 on TLC. The active principle in its purified form is a white powder of reel ting ...
With peak of hesperidin as the reference, HPLC fingerprints of 10 batches of Citri Reticulatae Pericarpium Viride were determined. The similarity of the 10 batches of samples was evaluated by Similarity Evaluation System for Chromatographic Fingerprint of TCM(2012 edition) to determine the common ...
Out of the 5 peak fractions (P1鈥揚5) obtained from normal phase HPLC of the Mc2-Ac-hex, only peak fraction 3 (P3) induced differentiation of HL-60 cells as evident from an increase in NBT-positive cells and increased expression of cell surface marker CD11b. The P3 differentiated the ...
Seventeen chromatographic peaks were selected as characteristic peaks and their relative peak areas (RPA) were calculated for quantitative expression of the HPLC fingerprints. The correlation coefficients of similarity in chromatograms were higher than 0.95 for the same species while much lower than 0.6...