To measure UV Spectroscopy scientists rely on spectrophotometers, sophisticated instruments that record the number of photons that reach the detector when directed through a sample. This allows scientists to determine the light intensity of a substance and determine its concentration....
Principle of Spectrophotometer The spectrophotometer technique is to measure light intensity as a function of wavelength. It does this by diffracting the light beam into a spectrum of wavelengths, detecting the intensities with a charge-coupled device, and displaying the results as a graph on the de...
Figure 1: A simplified schematic of the main components in a UV-Vis spectrophotometer. Credit: Dr. Justin Tom. Light source As a light-based technique, a steady source able to emit light across a wide range of wavelengths is essential. A single xenon lamp is common...
The UV Spectrophotometerrevealed the quantitive formation of silver nanorods. The charecterstics of obtained silver nanorods were studied using Fourier Transform Infrared Spectroscopy (FT-IR), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analysis. Antibacterial activity of ...
A photometer is also used in the spectrophotometer. The photometer detects the amount of intensity of light. It includes a detector or digital display. Spectrophotometer Principle The spectrophotometer can be operated in the UV region, Visible spectrum and IR spectrum as well. This instrument is ...
Both DNA and RNA have an absorbance maximum at 260 nm from which their concentration can be determined. The purity of the nucleic acid can also be assessed from the ratio of absorbance readings at specific wavelengths. You’ve just watched JoVE’s introduction to the spectrophotometer. ...
The molar extinction coefficient is part of the concept beind a spectrophotometer. The molar extinction coefficient describes the ability of a specific type of chemical to absorb light at a certain wavelength. This value is constant for whatever the specified wavelength is....
Densitometers:Determine the density of a material. Spectrophotometers:Measure the spectral reflectance and transmittance of a surface. Tristimulus colorimeter:Employed to measure the tristimulus values of a color. ColorimeterOperating Procedure 1. Switch the device on by rotating the Power Switch knob in...
5. How many kinds of spectrophotometers do you have? Our spectrophotometer is divided into visible spectrophotometer, UV-Visible spectrophotometer, double beam spectrophotometer. 6. Where is the spectrophotometer used? Can be applied to factories, schools, agriculture, food, bioch...
A solution of ibuprofen of 206 mg/L in 0.1 M phosphate buffer solution was treated with different amounts of fresh, electrochemically synthesized ferrate(VI). The changes of ibuprofen concentration in samples were determined using a UV-Vis spectrophotometer. The extent of mineralization was estimated...