Column chromatography is a technique in which the substances to be separated are introduced onto the top of a column packed with an adsorbent, passed through the column at different rates that depend on the affinity of each substance for the adsorbent and for the solvent or solvent mixture, and...
High-performanceliquid chromatography or commonly known as HPLC,is an analytical technique used to separate, identify or quantify each component in a mixture. The mixture is separated using the basic principle of columnchromatographyand then identified and quantified by spectroscopy. In the 1960s, the...