Primer sequences used for RT-PCR.Rong, Yao
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sequenceRT-PCRexpressionUsing oligonucleotide primers based on mammalian nitric oxide synthases (NOS), expression of an inducible NOS (iNOS) gene was ... KJ Laing,LJ Hardie,W Aartsen,... - 《Developmental & Comparative Immunology》 被引量: 205发表: 1999年 A zebrafish(danio rerio) model of ...
aAnswer: The Primer sequences for real-time PCR and nMS-PCR have supplied in Table 1 and Table 2 and the antibodies information in Methods of Western blot have revised in the materials and methods section in the profile of supplement methods. 答复: 底漆序列为实时PCR和nMS-PCR在表1供应了,...
The PrimerQuest Tool is the program of choice for designing qPCR primers/probes, sequencing oligonucleotides, and custom primers. Here is how to select the best PCR/qPCR assays for your experiments. Step 1: Enter sequence(s) The PrimerQuest Tool can perform batch analyses with up to 50 sequ...
Primers used in RT-PCR analyses in E are indicated with half arrows; their nucleotide sequences can be found in Supplemental Table S1. E, RT-PCR ... X Zheng,ND Miller,DR Lewis,... 被引量: 0发表: 0年 Serum microRNA...
7.打开PCR引物设计网站Primer3 Input (version 0.4.0) 将刚才找到了蛋白质翻译区的碱基序列复制到下图的框内。 8.将Product Size Ranges PCR产物长度一般控制在100-250,Primer GC%控制在40到60之间,最佳为50。如图。最后点击pick primers开始电脑设计引物。
for real-time PCR. Sequences of primers for real-time PCR.Sequences of primers for real-time PCR.Ci, ChenLiang, ShenShiping, CaoXixian, LiWanling, XuanJingwen, ZhangXiaobo, HuangJianping, BinDingli, XuGuofeng, Li
Comparing the sequence data of the internal transcribed spacer (ITS) regions of these fungi with each other and with those from the database, we designed four pairs of polymerase chain reaction (PCR) primers for each fungus. The primers allowed amplification of the DNA of the objective fungi...
4、d primer sequences for PCR amplification and sequencing from nuclear ribosomal RNA ()Vilgalys lab, Duke University Over the years, our lab has compiled a useful list of conserved primer sequences useful for amplification and sequencing of nuclear rDNA from most major groups of fungi (primarily...