5×prime script buffer 这个代表你给扩增的DNA一个反应环境,Prime script RT Enzyme mix 这个是原料混合液,就是组成DNA所需的A T C G,RNase Free H2O 这个是RBA酶不含水。Total RNA这个是你要扩增的目的基因。 查看完整答案 结果3 举报 逆转录聚合酶链反应,或变形,被广泛用于聚合酶链反应(PCR),反转录PCR...
MBI K0182 Long PCR Enzyme MixMBI K0191 High Fidelity PCR Enzyme MixMBI K0192 High Fidelity PCR Enzyme MixMBI K0201 Long PCR Control SetMBI K0211 GeneJET™ Fast PCR Master Mix (2X)MBI K0411 T7 Transcription KitMBI K0412 T7 Transcription KitMBI K0421 ExoIII/S1 Deletion KitMBI K0501 ...
Fermentas K0181 Long PCR Enzyme Mix 100 units 284 Fermentas K0182 Long PCR Enzyme Mix 500 units 1,136 Fermentas K0191 High Fidelity PCR Enzyme Mix 100 units 340 Fermentas K0192 High Fidelity PCR Enzyme Mix 500 units 1,363 Fermentas K0211 PyroStart™ Fast PCR Master Mix (2X) 250...
For example, the additional sequence may be a restriction enzyme recognition sequence. In certain aspects of the invention, the oligonucleotide further comprises a spacer sequence positioned between the hybridization sequence of length n and the promoter recognition sequence. In certain aspects of the ...
AgPath-ID One-Step RT-PCR Kit (Life Technologies, Carlsbad, CA) constituting ArrayScript reverse transcriptase, AmpliTaq Gold DNA polymerase, and buffer, was utilized for qRT-PCR. Each 25 μL reaction consisted of 0.09 μL H20, 12.5 μL of 2X reaction mix buffer, 1 μL 25X...