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Contact Primeco Education today to book Coach Behnam for your next Organization Development needs, team training and future program planning, speaking events, or your coaching and consulting needs. Primeco Education’s wide range of programs, events, and coaching products are designed to transform yo...
mRNA products were precipitated in 2.5 M lithium chloride, washed twice with 70% ethanol, dissolved in nuclease-free water and stored at −80 °C. Nucleofection of APOE4 murine astrocytes Astrocytes containing APOE4 isoform of the APOE gene (originated from Taconic Biosciences) were nucleo...
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The ligation products were purified with Monarch PCR & DNA Cleanup Kit and electroporated into MegaX DH10B T1R Electrocomp Cells (Thermo Fisher). The bacteria were grown overnight in liquid culture and plasmid was extracted using the Plasmid Plus Midi Kit. The pegRNA sequences are shown in...
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