In contrast, α16-α20 of Cps7D’s TPR domain superimpose with α11-α15 (orange) of the equivalent protomer 1 of the Cps3D dimer. An arrow labeled ‘TPR shift’ indicates that α1’-α15’ (light blue) and α11-α15 (orange) belong to two different protomers of the Cps3D ...
RNA was extracted from ESCs using a Zymo Research (Orange, CA, USA, Proteingene, France) kit. One microgram of RNA was reverse transcribed using the SuperscriptII reverse transcriptase (Invitrogen, Cergy, France) and oligo(16)dT. Quantitative PCR was performed using a Light Cycler LC 1.5(...