Most short sequences can be precisely written into a selected genomic target using prime editing; however, it remains unclear what factors govern insertion. We design a library of 3,604 sequences of various lengths and measure the frequency of their insertion into four genomic sites in three hum...
Default parameters were used to estimate library size factors, gene-wise dispersion and fitting of the negative binomial GLM to determine log2 fold change values. The log fold change shrinkage was performed using the apeglm algorithm (1.22.1)57. The default two-sided Wald test was used to ...