Structure of a bifunctional DNA primase-polymerase. Nat. Struct. Mol. Biol. 11 (2), 157-162.G. Lipps, A.O. Weinzierl, G. von Scheven, C. Buchen, P. Cramer, Structure of a bi- functional DNA primase-polymerase, Nat. Struct. Mol. Biol. 11 (2004) 157-162....
Here we present a structure-function analysis of the pRN1 primase-polymerase (prim-pol) domain. The crystal structure shows a central depression lined by conserved residues. Mutations on one side of the depression reduce DNA affinity. On the opposite side of the depression cluster three acidic ...
DNA synthesisDNA repairDNA primersMOLECULAR structureCRYSTAL structurePOLYMERASESCells utilise specialized polymerases from the Primase-Polymerase (Prim-Pol) superfamily to maintain genome stability. Prim-Pol's function in genome maintenance pathways including replication, repair and damage tolerance. ...
Sequence analysis and phylogeny suggested gp577 to be a primase-polymerase (PrimPol)—the first PrimPol to be identified in a nucleocytoplasmic large DNA virus (NCLDV). Recombinant gp577 protein purified as a homodimer and exhibited de novo RNA as well as DNA synthesis on circular and linear ...
A primase activity associated to DNA polymerase alpha from rat liver is described. Both activities were absent in normal adult rat liver but were concomitantly induced after partial hepatectomy. As previously shown for polymerase alpha and DNA topoisomerase II, primase activity reached a maximum value...
1) DNA primase polymerase α complex DNA引物酶-多聚酶α复合体1. Methods:DNA primase polymerase α complex was extracted and purified from EAC cells at 4℃. 目的:研究碘化-3,3'-二乙基-9-甲基-硫杂羰花青(3,3'-Diethyl-9-methylthiacarbocyanineiodide;DMTCCI)对DNA引物酶-多聚酶α复合体的...
Gain an understanding of DNA polymerase and RNA polymerase. Learn what DNA polymerase does and explore the functions of RNA primase and their end products. Updated: 11/21/2023 Table of Contents What is DNA Polymerase? What is RNA Polymerase? Topoisomerase Lesson Summary Frequently Asked Questions...
The role of the cellular DNA polymerase alpha-primase holoenzyme was probed by using soluble extracts from rodent cells that replicate bovine papilloma virus 1 and human papilloma virus 6b DNA in the presence of the viral E1 helicase and the E2 transcription factor. Monoclonal antibodies directed ...
The DNA polymerase -primase synthetic activity was specifically stimulated by hGINS on various primed DNA templates. The significance of these findings is discussed in view of the molecular dynamics at the human replication fork. 展开 关键词: DNA polymerase DNA primase DNA replication genome dynamics...
Using purified Mcm and DNA primase complexes, a direct physical interaction is detected in pull-down assays between the Mcm2∼7 complex and the hetero-dimeric DNA primase composed of the p48 and p58 subunits. The Mcm4/6/7 complex co-sediments with the primase and the DNA polymerase α-...