DNA primase is a specialized DNA-dependent RNA polymerase, which is capable of synthesizing a short (10nt) RNA strand starting from a single-stranded DNA as a template. From: Brenner's Encyclopedia of Genetics (Second Edition), 2013
Augustin MA, Huber R, Kaiser JT (2001) Crystal structure of a DNA-dependent RNA polymerase (DNA primase). Nat Struct Biol 8(1):57–61Augustin,M.A., Huber,R. and Kaiser,J.T. (2001) Crystal structure of a DNA-dependent RNA polymerase (DNA primase). Nat. Struct. Biol., 8, 57-61...
Primase is the ssDNA-dependent RNA polymerase that synthesizes RNA primers during DNA replication. In common with all DNA and RNA polymerases, primase has structural and functional features involved in polymer elongation. As RNA polymera... MA Griep - 《Indian Journal of Biochemistry & Biophysics》...
T7 primase, which can synthesize RNA primers, is located in the N-terminal half of the multifunctional gene 4 helicase-primase protein13. The primase domain is composed of an RNA polymerase domain (RPD) and a zinc-binding domain (ZBD). The two domains are connected by an unstructured ...
DNA replication is heavily dependent on several enzymes to progress. An example of these enzymes is DNA polymerase. DNA polymerase is found in both eukaryotes and prokaryotes and is a highly conserved structure. This means that the active site of this enzyme, which is where it processes the ...
DNA replication in almost all organisms depends on the activity of DNA primase, a DNA-dependent RNA polymerase that synthesizes short RNA primers of defined size for DNA polymerases. Eukaryotic and archaeal primases are heterodimers consisting of small catalytic and large accessory subunits, both of...
Pol-prim is comprised of two differentpolymerases,primaseand pol α, whose activities are distinct but tightly coordinated. Primase is a DNA dependentRNApolymerasethat initiates daughter strand synthesis by generating the initial primer of 7–12ribonucleotides(NTPs). This initial RNA primer is transfer...
Therefore, it is warranted to better investigate the biological and molecular processes in HCC and provide novel targets for HCC. DNA primases synthesize primers for DNA polymerases-mediated DNA replication. DNA primases are also defined as DNA-dependent RNA polymerases. The polymerases complex ...
Further, gp577 extends a DNA/RNA primer annealed to a DNA or RNA template using deoxyribonucleoties (dNTPs) or ribonucleotides (NTPs) demonstrating its DNA/RNA polymerase and reverse transcriptase activity. We also show that gp577 possesses terminal transferase activity and is capable of ...
Replicative DNA polymerases cannot initiate DNA synthesis de novo and rely on dedicated RNA polymerases, primases, to generate a short primer. This primer is then extended by the DNA polymerase. In diverse archaeal species, the primase has long been know