1) primary mouse embryonic fibroblast 原代小鼠胚胎成纤维细胞 2) mouse embryo fibroblasts 小鼠胚胎成纤维细胞 1. Objective To research the productive infection ofmouse embryo fibroblastswith human cytomegalovirus(HCMV) in vitro and to research the relationship between cell surface heparan sulfate and the...
Mouse embryonic fibroblasts (MEFs) are commonly collected as a means to maintain the culture and growth of embryonic stem cells (ESCs). However, their utility can extend well beyond their use exclusively in ESC culture. With collection from various transgenic mouse models, use of MEFs may serve...
Freshly prepared primary mouse embryonic fibroblasts (MEFs, strain DR4, non-irradiated) were obtained from Genome Manipulation Core at the Salk Institute. Primary MEFs were grown in DMEM supplemented with 10% FBS and 0.1 mM non-essential amino acid (#11140-050; invitrogen) in a 37 °C ...
biocompatibility with primary mouse embryonic fibroblast (PMEF) cells. Chen et al. [40] cultured PC12 cells with carbon nanotubes and rGO films with the same initial seeding density for 5 days, and the results suggest that the cells cultured with rGO enhanced proliferation, whereas nanotubes inhi...
Using the second set of sterile instruments and a fresh sterile petri dish, isolate the embryos. Be sure to remove the placement and embryonic sacs. Using the scalpel handle with the #11 blade on it, cut off embryo heads and scoop out the liver with a pair of forceps. The head and for...
We sought to investigate this mechanism in a normal cell background using isogenic normal primary culture cells derived from mouse embryonic fibroblasts (MEF) with varied genomic status for Egr-1 gene (cells with both intact Egr-1 alleles,Egr-1 +/+; cells with homozygous deletion ofEgr-1 ...
Fast and efficient transfection of mouse embryonic stem cells using non-viral reagents. Stem Cell Rev. Rep. 12, 584–591 (2016). Article CAS PubMed Google Scholar Chen, S. X. et al. Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series...
Mouse embryonic fibroblasts: Mouse embryonic fibroblasts were isolated from E13.5 embryos using the WiCell protocol (WiCell Research Institute, Madison, WI). Cells were cultured in DMEM, 10% FBS, Pen/Strep, and l-Glut. Before senescence occurred, cells were harvested and nucleofected in triplicate...
Primary Mouse Embryonic Fibroblasts Primary Mouse Mammary Epithelial Cells Primary Mouse Proximal Tubule Cells Primary Movements Teaching System Primary Mucosal Malignant Melanoma of the Larynx Primary Multi-Function Display Primary Multifocal Leptomeningeal Gliomatosis ...
Primary mouse embryonic fibroblasts were prepared as previously described51. Briefly, on gestation Day 13.5, pregnant female mice were killed and embryos were surgically separated, decapitated, and eviscerated. The bodies were minced, digested in trypsin for 10 min, and cells were plated in T-75...