After centrifuga- tion at 10 000 Â g for 15 min at 4 1C, the supernatant was fractionated on an 8–16% sodium dodecyl sulfate–polyacrylamide gel electrophoresis gel and proteins were transferred to a nitrocellulose membrane (Thermo Scientific, Lafayette, CO, USA). The membrane was ...
Control transfections were performed using 10 nM ON-TARGETplus Non-targeting siRNAs (D-001810-10, Dharmacon, Lafayette, Colorado, USA). The siRNAs were incubated in Opti-MEM medium for 30 minutes at room temperature. The siRNA mix was then added to the wells and cell suspension was ...