Cell TransplantationDavis TA, Black AT, Kidwell WR, Lee KP. Conditioned medium from primary porcine endothelial cells alone promotes the growth of primitive human haematopoietic progenitor cells with a high replating potential: evidence for a novel early haematopoietic activity. Cytokine. 1997; 9 :...
Of 300 cells plated, 29 clones were obtained after two weeks and all clones were positive for CD31. To evaluate the specificity and recovery efficiency of this method, the CD31+ murine endothelial cell line H5V was mixed with a melanoma cell line and/or mixed with L929 fibroblasts. In both...
enabling passive medium flow at freely chosen time points. In contrast to our earlier work, the new lung-on-chip is equipped with passive medium exchange that enables not only the reproduction of the cyclic mechanical stress, but also long-term cell culture ...
5. Using aseptic technique, and working in a laminar flow hood or biosafety cabinet, transfer the volume of each growth kit component, as indicated below, to the bottle of basal medium using a separate sterile pipette for each transfer. a. If using the Endothelial Cell Growth Kit-BBE (BioVe...
PropagationUse of PriCoat™ T25 Flasks (G299) or . Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions.The base medium for this cell line is Prigrow X series medium available...
Primary Cells from their source organ tissue and media optimized for each individual cell type (and always in stock).
These primary cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500) and subsequently grown and passaged in Cell Systems Complete Classic Medium (4Z0-500). The cells are cryopreserved in Cell Systems Cell Freezing Medium (4Z0-705). Isolated from normal, healthy...
An aliquot of the fresh cell suspension is transferred to a new culture flask that already contains warm growth medium. If the aliquot is 1mL, which is 1/8 of the cell suspension created in the previous step, then the cells are said to have undergone a 1:8 split. If a culture is be...
Addition of heparin and endothelial cell growth supplement to the medium enhanced proliferation. The endothelial origin of the cultured cells was confirmed by immunofluorescent demonstration of the presence of Factor VIII-related antigen and angiotensin-converting enzyme. In addition, tight junctions ...
Human umbilical vein endothelial cell (HUVEC) cell culture and transfection. Primary HUVEC were obtained from Lonza and cultured on fibronectin-coated culture flasks in EGM-2 medium, supplemented with singlequots (Lonza, Verviers, Belgium). HUVEC at passage #4 or #5 were transfected with 2...