ÁngelGaudioso, ...María DoloresLedesma, inAdvanced Drug Delivery Reviews, 2022 3.1Primary cell cultures Primary cell cultureis defined as theex vivoculture of cells freshly obtained from a tissue. The main advantages of primary cells are their faithfultranscriptomicandproteomicprofile and closer prox...
展开 关键词: Humans Cells, Cultured Histocompatibility Antigens Class II Capillary Permeability Cerebrovascular Circulation Cell Division Microcirculation Microscopy, Electron, Scanning Interferon-gamma Endothelium, Vascular DOI: 10.1007/BF02899279 被引量: 183 年份...
ObjectivePrimary human preadipocytes and differentiated adipocytes in culture are valuable cell culture systems to study adipogenesis and adipose function in relation to human adipose biology. To use these systems for mechanistic studies, siRNA-mediated knockdown of genes for its effectiveness was studied....
Development of long-term cell culture protocols for analyzing the effect of chronic antigenic stimulation on primary T lymphocytes from young adult donors has led to the identification of multiple characteristics of the terminal stage of replicative senescence in this cell type. The complex changes ...
A cohort of genes was selected to characterize the adipogenic phenotype in primary cell cultures from three tissue sources. We compared the quantitative expression of biomarkers in culture relative to their expression in vivo because the mere presence or absence of expression is minimally informative. ...
Note:Should not be used for therapeutic or diagnostic purposes in humans or animals. Selection conditions of animal cells: Suitable for from the first generation to the tenth generation within the cultivation of stem cells and delicate cells.It is recommended to use 10% of the concentration. Plea...
Monolayers also expressed net secretory transport of urate. This cell culture preparation may provide a useful working model for the study of avian PT transport. 展开 关键词: electrophysiology electron microscopy sodium-glucose luminal transporter amiloride urate ...
However, the biggest drawback of primary HAE models is their limited proliferative lifespan in culture. Much work has been devoted to understand the factors, which govern the HAE cell proliferation and differentiation, both in vivo and in vitro. Here, I have summarized recent achievements in ...
In the LUHMES cell model [26], we used unsynchronized neuronal precursor cell proliferation conditions and timed (day 0/day 1; d0/d1), change of culture medium-induced release into neuron differentiation. We noticed that neurons with detectable cilia more efficiently accomplished axon outgrowth as...
216,217 The vaccine thus developed, the human diploid cell vaccine (HDCV), containing concentrated and purified virus, evoked much better immune responses in experimental animals and in humans than did DEV, suckling mouse brain, or adult brain tissue vaccines.218,219 The technical advances leading...