First Medical Associates’ Primary Care Centers are dynamic, modern practices delivering high-quality, individualized primary care in multiple convenient locations in Rockville, Germantown, Gaithersburg, Columbia, Bowie, Nottingham, Frederick, Severna Park, and Silver Spring, Maryland. We provide a compreh...
CMV PCR was performed from 200 µL of serum. For nucleic acid extraction, the automated system QIASymphony (Qiagen, Germantown, MD, USA) with the DSP virus/pathogen minikit was used following manufacturer recommendations. For amplification and detection, the Rotor-Gene Q (Qiagen) with Artus®...
affinity chromatography (Qiagen Sciences, Germantown, MD, USA). Prior to use, each protein was further purified by reverse phase high performance liquid chromatography (HPLC) to yield endotoxin-free protein [16]. Levels of endotoxin were <0.05 endotoxin units (<5 pg) per mg of recombinant prote...