Milford, MA, USA), using a Superdex 200 HR10/30 column (GE Healthcare). Samples were loaded onto the column and eluted in an isocratic manner with 50 mM Bis-Tris-HCl (pH 7.5) containing 0.2 M NaCl, at a flow rate of 0.75 ml/minute. Separated protein fractions were ...
Animal studies were performed with the approval of the University of Pittsburgh Institutional Animal Care and Use Committee and conducted according to the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Animals were housed in a temperature- and humidity- controlled ...
MD Andersons’s Institutional Animal Care and Use Committee approved all procedures for animal studies. Xenografts with the D423 (MTAP-WT), U87 (MTAP-deleted), U87 pCMV MTAP (MTAP-rescued), NB1 (MTAP-WT), SKMEL5 (MTAP-deleted), and Gli56 (MTAP-deleted) cancer cell lines were gen...
The isoelectric focusing step was performed on an Ettan IPGphor 3 (GE Healthcare) and consisted of a 1 h step at 150 V. Voltage was then increased from 150 to 1000 V in 1 h and then increased from 1000 to 2000 V during 1h. A constant voltage of 2000 V was then applied until ...
International Journal of Molecular Sciences Article Low Levels of IgG Recognizing the α-1-Antitrypsin Peptide and Its Association with Taiwanese Women with Primary Sjögren's Syndrome Yu-Sheng Chang 1,2, Chih-Hong Pan 3,4, Che-Chang Chang 5 ID , Kai-Leun Tsai 1, Han-Wen Chou 6, Jin-...