First Medical Associates’ Primary Care Centers are dynamic, modern practices delivering high-quality, individualized primary care in multiple convenient locations in Rockville, Germantown, Gaithersburg, Columbia, Bowie, Nottingham, Frederick, Severna Park, and Silver Spring, Maryland. We provide a compreh...
Gaithersburg, MD: Aspen. Google Scholar Westchester County Social Competence in the Schools Task Force (1990).Promoting social development in elementary school children: A task force report. Westchester County, NY: Department of Community Mental Health. Google Scholar White, B. (1988).Educating ...
Pregnant women may receive any licensed, recommended, age-appropriate influenza vaccine. Afluria (IIV3; Seqirus, Parkville, Victoria, Australia) may be used for persons aged ≥5 years, consistent with Food and Drug Administration–approved labeling. FluMist Quadrivalent (LAIV4; MedImmune, Gaithersbur...
Briefly, 200 ng totalRNA were reverse transcribed using qScript cDNA Super Mix (Quanta Biosciences, Gaithersburg, MD) following the manufacturer's instructions. After completion of cDNA synthesis 1/10 of the ® ®first strand reaction were used for probes TaqMan Gene Expression PACssRayasm(...
The cells were cultured in complete growth media (CGM) composed of Leboeuf's DMEM (Quality Biochemicals, Gaithersburg, MD, USA) at pH 6.7, including 20% FBS (ThermoFisher), 1% GlutaMAX™ Supplement (ThermoFisher), and penicillin-streptomycin (50 U/mL, ThermoFisher), and incubated at ...
Briefly, adult mice were euthanized with isoflurane and all DRGs were collected in cold Neurobasal Medium (Gibco/ThermoFisher Scientific) with 10% fetal bovine serum (JR Scientific, Woodland, CA), 100 units/ml Penicillin, 100 μg/ml Streptomycin (Quality Biological, Gaithersburg, MD) and ...
Alkaline comet assay was performed using CometAssay Kit (Trevigen, Gaithersburg, MD, USA). Primary SMCs were detached and resuspended in cold PBS to a concentration of 105 cells/mL. An aliquot of 10 μL of the cell suspension was mixed with 100 μL of melted LM agarose (37 °C). The...
(Cat: 25-047-100, Quanta Biosciences, Gaithersburg, MD, USA). Quantification of gene expression was performed on 1ng μl−1cDNA per well, 200 nMof each primer with KAPA SYBR FAST qPCR Kit (Cat: KK4602, Kappa Technologies, Woburn, MA, USA) using QuantStudio 12 Flex System (Applied...
(50-AATTCT CCGAACGTGTCACGT-30).36 107 T lymphocytes, resuspended in 100 ml of the Amaxa Nucleofector kit solution (Lonza, Walkersville, MD, USA), were electro- porated with 5 mg of the indicated siRNA using the V-024 program of the Nucleofector (Amaxa Biosystems, Gaithersburg, MD, USA...
The pmaxGFP plasmid (AMAXA Biosystems, Gaithersburg, MD) that contains an enhanced green fluorescent protein (EGFP) gene under regulation of a cytomegalovirus (CMV) enhancer/promoter element and is kanamycin resistant, was then added (2 μg/transfection sample) to the cell suspension. The cell/...