Chinese soft-shelled turtlePelodiscus sinensis(P.sinensis), belonging to the Reptilia, Testudines, turtle family, is an important aquaculture species in southern China. This species exhibits a sex-dependent dimorphic growth pattern. Compared with females, male individuals are characterized by larger body...
There is still an international problem of overuse of antibiotics for self limiting disease What happens in the consultation room makes a difference to patient understanding and ability to cope. Strategy: An understanding of parental perceptions is needed to plan a strategy of culture change around ...
The bacteria were grown in LB broth (Becton Dickinson, USA) with agitation at 37°C using appropriate antibiotics whenever applicable. For bacterial enumerations, Brilliant Green Agar plates (Becton Dickinson, USA) were used. Table 1. List of bacterial strains, plasmids, and primers used in ...
Cells were cultured in MEM/199 medium (Invitrogen) containing 5 mM glucose, 10% horse serum, 10% fetal calf serum, 0.5% Ultroser G, antibiotics and 2 mM l-glutamine for myoblasts proliferation. Cells were transfected with lipofectamin 2000 (Invitrogen). Six hours after transfection, cells were...
Cells were subsequently stimulated for the indicated time-points with either TGFβ1 (2 ng/ml) or TNFα (10 ng/ml) in DMEM/F12 medium supplemented with 0.1% FCS and antibiotics. Supernatants were collected and cells were washed with cold PBS and thereafter taken for RNA purification. ...
The images were analyzed by using Image QUANT TL8.1 (GE Healthcare, Marlborough, MA, US). Cell culture, transfection and cell viability assay Cal27 and HSC3 cells were maintained in DMEM or EMEM, respectively, containing 10% fetal bovine serum, antibiotics (100 U/mL penicillin and 100...
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