Gateway entry vectors were used to generate different combinations of mouse Multicilin tagged with 3xFLAG, mouse E2f4 tagged with a NLS and a 6xmyc epitope, and mouse E2f4VP16 tagged with NLS and a 6xmyc epitope, using a combination of PCR along with Topo and Gibson-based cloning strategie...
However, less is known about the basal foot structure and composition in primary and motile cilia, and no consensus has yet been reached on its nomenclature, such that this assembly has been named differently depending on the study and cell type analyzed (e.g., satellite arms, Sorokin, 1968...