Assay protocol 1. Add cells in appropriate medium to microplate wells 2. Add either PrestoBlue HS or PrestoBlue reagent to microplate wells (see recommended volumes) 3. Incubate at 37ºC for 10 minutes 4. Read fluorescence or absorbance (signal is ...
PrestoBlue HS has a larger assay window compared to PrestoBlue The PrestoBlue HS reagent displays a larger viability detection window for various cell lines allowing for improved quantitative measurement of cell viability (Figure 6). Click image to enlarge ...
Note: Correct for background fluorescence by including control wells containing only cell culture media (no cells) on each plate. 2. Incubate ≥10 minutes at 37º C. Longer incubation times will increase sensitivity of detection. As this is a live cell assay, readings may be taken at multi...
This in vitro study compares the sensitivity of a new, high-sensitivity PrestoBlue (hs-PB) assay with standard PrestoBlue (PB) in assessing the efficacy of valinomycin and antimycin A in human vascular endothelial EA.hy926 cells, as well as cell viability. Methods The metabolic activity of EA...
Note: Correct for background fluorescence by including control wells containing only cell culture media (no cells) on each plate. 2. Incubate ≥10 minutes at 37º C. Longer incubation times will increase sensitivity of detection. As this is a live cell assay, readings may be taken at multi...
PrestoBlue HS has a larger assay window compared to PrestoBlue The PrestoBlue HS reagent displays a larger viability detection window for various cell lines allowing for improved quantitative measurement of cell viability (Figure 6). Click image to enlarge ...
Other than the Invitrogen alamarBlue Cell Viability Reagent, AlgiMatrix Matrix is also compatible with Invitrogen PrestoBlue Cell Viability Reagent and Invitrogen CyQuant Direct Cell Proliferation Assay. The protocol is almost the same as the 2D cell culture, but it needs the blank AlgiMatrix Matrix ...
PrestoBlue HS has a larger assay window compared to PrestoBlue The PrestoBlue HS reagent displays a larger viability detection window for various cell lines allowing for improved quantitative measurement of cell viability (Figure 6). Click image to enlarge ...
Figure 6. Increased dynamic range with PrestoBlue HS reagent.Various concentrations of gambogic acid were added to A549 cells and the changes in viability were detected. The results demonstrate a significant improvement in the dynamic range of the assay whe...
PrestoBlue Cell Viability Stain and CyQUANT Cell Proliferation Assay Kit can be used. We also offer a Neurite Outgrowth Staining Kit (Cat. No. A15001). More information about our different assays for neuronal cell health can be found here (https://www.thermofisher.com/us/en/home/life-science...