Assay protocol 1. Add cells in appropriate medium to microplate wells 2. Add either PrestoBlue HS or PrestoBlue reagent to microplate wells (see recommended volumes) 3. Incubate at 37ºC for 10 minutes 4. Read fluorescence or absorbance (signal is...
Continuously monitor the effects of drugs in dose response assay or when optimizing drug concentrations Perform other functional assays after cell viability measurements with PrestoBlue HS or PrestoBlue are complete PrestoBlue HS has improved performance ...
Note: Correct for background fluorescence by including control wells containing only cell culture media (no cells) on each plate. 2. Incubate ≥10 minutes at 37º C. Longer incubation times will increase sensitivity of detection. As this is a live cell assay, readings may be taken at multi...
This in vitro study compares the sensitivity of a new, high-sensitivity PrestoBlue (hs-PB) assay with standard PrestoBlue (PB) in assessing the efficacy of valinomycin and antimycin A in human vascular endothelial EA.hy926 cells, as well as cell viability. Methods The metabolic activity of EA...
Additionally, the contaminating resorufin and the resulting higher background signal to help reduce the signal-to-background ratio and dynamic range of the assay. To improve the performance of the resazurin-based reagents, an innovative process was developed...
Additionally, the contaminating resorufin and the resulting higher background signal to help reduce the signal-to-background ratio and dynamic range of the assay. To improve the performance of the resazurin-based reagents, an innovative process was developed...
Assay protocol 1. Pre-incubate or dispense cells in appropriate microplate wells 2. Add PrestoBlue 10X reagent to microplate wells (see recommended volumes) 3. Incubate at 37°C for 10 minutes 4. Read fluorescence at 590 nm 5. Add CyQUANT ...
NucRed Dead 647 ReadyProbes Reagent Protocol for Viability PrestoBlue Assays for Cell Viability Protocol, for Microplates PrestoBlue and CyQUANT Direct Confirmation Assay for Cell Viability ReadyProbes Cell Viability Imaging Kit, Blue/Green ReadyProbes Cell ...
PrestoBlue HS has a larger assay window compared to PrestoBlue The PrestoBlue HS reagent displays a larger viability detection window for various cell lines allowing for improved quantitative measurement of cell viability (Figure 6). Click image to enlarge ...
PrestoBlue HS has a larger assay window compared to PrestoBlue The PrestoBlue HS reagent displays a larger viability detection window for various cell lines allowing for improved quantitative measurement of cell viability (Figure 6). Click image to enlarge ...